Volume: 41 Issue: 2
Year: 2010, Page: 40-42, Doi: https://doi.org/10.51966/jvas.2010.41.40-42
Received: July 1, 2009 Accepted: Feb. 16, 2010 Published: Dec. 31, 2010
A study was conducted to find out the antigenic similarity of immunoglobulin G (IgG) of sambar deer and chital to that of goats, employing enzyme linked immunosorbant assay (ELISA). Various dilutions of goat, sambar deer, chital and guinea pig sera were reacted against various dilutions of antigoat (AG) horse radish peroxidase (HRP) conjugate and the optical density values (OD) measured. Results of the study indicated that there was cross reactivity between deer and goat immunoglobulins which was evidenced by colour development in ELISA. It was also observed that the AG HRP conjugate detected chital IgG to a greater extent than sambar deer IgG. However, statistically there was significant difference between the optical OD values for the four species. It is concluded that there exists antigenic similarity between deer and goat IgG and that deer IgGs differ among themselves in their capacity to bind with AG HRP conjugate
Keywords: Immunoglobulin G, sambar deer, chital, goat, ELISA