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Journal of Veterinary and Animal Sciences

Volume: 56 Issue: 3

  • Open Access
  • Research Article

Effect of dietary supplementation of Bacillus subtilis, Lactobacillus plantarum and mannan oligosaccharides on immune response in broiler chicken

Shamna, T.P.1, Binoj Chacko1, Stella Cyriac2, Simi, G.1, Radhika, G.3 and Deepa Jolly4

1Department of Poultry Science, College of Veterinary and Animal Sciences, Mannuthy, Thrissur, 2Department of Poultry
Science, CASM, Thiruvazhamkunnu, 3Department of Animal Genetics and Breeding, College of Veterinary and Animal
Sciences, Mannuthy, Thrissur, 4Department of Veterinary Public Health, College of Veterinary and Animal Sciences,
Mannuthy, Thrissur, Kerala Veterinary and Animal Sciences University, Pookode, Wayanad-673576, Kerala.

Year: 2025, Page: 418-423, Doi: https://doi.org/10.51966/jvas.2025.56.3.418-423

Received: Dec. 10, 2024 Accepted: Feb. 11, 2025 Published: Sept. 30, 2025

Abstract

A study was conducted to study the influence of dietary supplementation with Lactobacillus plantarum, Bacillus subtilis, mannan-oligosaccharides (MOS) and their combinations on intestinal immune response in broiler chicken. A total of 216-day-old Cobb broiler chicks were assigned to six treatment groups with each group comprising three replicates of 12 birds each, ensuring a completely randomised design (CRD): T1 (control), T2 (L. plantarum), T3 (B. subtilis), T4 (MOS), T5 (L. plantarum + MOS) and T6 (B. subtilis + MOS). The humoral immune response was assessed by measuring antibody titres against the Newcastle disease vaccine using the haemagglutination inhibition (HI) test at 7, 14, 21, 28, and 35 days of age. Significant differences in antibody titres were observed only on the 28th day, with the group receiving B. subtilis and MOS (T6) exhibiting the highest titre. The results suggest that B. subtilis, particularly in combination with MOS, enhanced the vaccine-induced immune response at specific time points. The cell-mediated immune response was evaluated through cutaneous hypersensitivity reactions to 2,4-dinitrochlorobenzene (DNCB) at 24, 48 and 72 hours post-injection, though numerical differences were noted, particularly in the T6 group at 48 hours, the variations were not statistically significant. The findings highlighted that dietary supplementation with probiotics and prebiotics had enhanced immune responses during specific periods but with consistent results. Future research may focus on optimising supplementation strategies and exploring mechanisms to maximize the immunomodulatory potential of probiotics and prebiotics in broilers.

Keywords: Bacillus subtilis, Lactobacillus plantarum, Mannan-oligosaccharide, immune response, broiler

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Cite this article

Shamna, T.P., Binoj Chacko, Stella Cyriac, Simi G, Radhika, G. and Deepa Jolly. 2024. Effect of dietary supplementation with Bacillus subtilis, Lactobacillus plantarum and mannan oligosaccharides on growth and livability in broiler chicken
J. Vet. Anim. Sci. 56 (3):418-423

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