Volume: 41 Issue: 2
Year: 2010, Page: 18-20, Doi: https://doi.org/10.51966/jvas.2010.41.18-20
Received: March 19, 2010 Accepted: June 7, 2010 Published: Dec. 31, 2010
Comparative efficacy of three tests viz., Ziehl-Neelsen acid fast staining of faecal smear, single intradermal Johnin test and IS900 faecal polymerase chain reaction were evaluated for diagnosis of paratuberculosis in goats. One hundred and fifty goats from the University Sheep and Goat farm, Mannuthy were subjected to single intradermal Johnin test. Faecal smears from all these goats were stained by Ziehl-Neelsen acid fast staining method. Faecal PCR specific for IS900 was also done on all samples. Among the three tests, IS900 PCR showed highest sensitivity in diagnosing paratuberculosis in goats and detected maximum number of infected animals. Single intradermal Johnin test and acid fast staining did not show any significant difference while acid fast staining of faecal smears detected least number of positive animals.
Keywords: Paratuberculosis, IS900 PCR, intradermal Johnin