Volume: 42 Issue: 2
Year: 2011, Page: 5-7, Doi: https://doi.org/10.51966/jvas.2013.42.5-7
Received: Sept. 12, 2011 Accepted: Oct. 21, 2011 Published: Dec. 31, 2011
Detection of antigens in faecal supernatants is a more reliable and suitable method, in cases where coprological examination fails to reveal any parasitic ova. Hundred cattle known to be infected with Gastrothylax crumenifer, the most prevalent amphistome species were selected at random for the study. An indirect ELISA was performed to detect coproantigens in faecal supernatants of these infected cattle using rabbit hyper immune serum against somatic antigens of the fluke. Seventy four samples were found to contain detectable amounts of coproantigens indicating a sensitivity of 74 per cent for the test. The results indicate the feasibility of ELISA for the detection of coproantigens of amphistome infections.
Keywords: Coproantigen, ELISA, Amphistomes