Volume: 56 Issue: 3
Year: 2025, Page: 424-428, Doi: https://doi.org/10.51966/jvas.2025.56.3.424-428
Received: Dec. 17, 2024 Accepted: April 2, 2024 Published: Sept. 30, 2025
Phospholipase C gamma 1 (PLCG1) gene is located on chromosome 13 of the cattle genome and consists of 32 exons. It plays a key role in calcium signalling pathways that contribute to disease resistance in cattle. This study was conducted to identify the polymorphisms in the PLCG1 gene in the native cattle of Kerala, namely the Vechur and Kasargod which are known for their disease resistance and adaptability to various agro-climatic conditions of the state. A 139bp fragment located in exon 31 of the PLCG1 gene on chromosome 13 was selected for analysis. High-Resolution Melting (HRM) analysis revealed homozygous CC, AA and heterozygote CA genotypes in both cattle groups. The CA genotype exhibited the highest frequency in both Vechur (0.66) and Kasargod (0.60) cattle. Chi-square analysis indicated that the Vechur cattle population was not in Hardy Weinberg Equilibrium (HWE) (p≤ 0.01), whereas, the Kasargod cattle population was in HWE (p≥0.05).
Keywords: PLCG1, Vechur cattle, Kasargod cattle, HRM
Bagnall, N., Gough, J., Cadogan, L., Burns, B. and Kongsuwan, K. 2009. Expression of intracellular calcium signaling genes in cattle skin during tick infestation. Parasite Immunol. 4: 177-187.
Chen, B., Cole, J.W. and Grond-Ginsbach C. 2017. Departure from Hardy Weinberg Equilibrium and genotyping error. Front Genet. 31:167. doi: 10.3389/fgene.2017.00167.
Cheng, H., Lyu, Y., Liu, Z., Li, C., Qu, K., Li, S., Ahmed, Z., Ma, W., Qi, X., Chen, N. and Lei, C.A. 2024. Whole-genome scan revealed genomic features and selection footprints of Mengshan cattle. Genes (Basel). 9: 1113.
Iype, S. 1996. The Vechur cattle of Kerala. J. Agric. 18: 59-78.
Iype, S. 2013. Vechur cattle–from extinction to sustainability. Anim. Genet. Resour. 52: 105-110.
Iype, S., Venkatachalapathy, T., Santosh, P.K. and Behera, A. 2016. Characterisation of Kasargod cattle of Kerala. IOSR J. Agric. Vet. Sci. 9: 36-47.
Khan, S.M., Faisal, A.R.M., Nila, T.A., Binti, N.N., Hosen, M.I. and Shekhar, H.U. 2021. A computational in silico approach to predict high-risk coding and non-coding SNPs of human PLCG1 gene. PLOS ONE. 16(11), p.e0260054.
Mandal, S., Bandyopadhyay, S., Tyagi, K. and Roy, A. 2021. Recent advances in understanding the molecular role of phosphoinositide-specific phospholipase C gamma 1 as an emerging onco-driver and novel therapeutic target in human carcinogenesis. Biochimica et Biophysica Acta (BBA)-Reviews on Cancer. 1876: 188619.
Patel, V.M. 2019. Functional interrogations of Phospholipase C Gamma 1 (PLCG1) mutations in Sezary Syndrome, Doctoral thesis, King’s College London. 255p. 2019_Patel_Varsha_Maheshkumar_0703260_ethesis.pdf
Sambrook, J. and Russell, D.W. 2001. Molecular Cloning: A Laboratory Manual (3rd Ed.). Cold Spring Harbor Laboratory Press, New York, 1886p.
Shen, W., Martinez, K., Chuang, C.C. and McIntosh, M. 2013. The phospholipase C inhibitor U73122 attenuates trans-10, cis-12 conjugated linoleic acid-mediated inflammatory signaling and insulin resistance in human adipocytes. J. Nutr. 143: 584-590.
© 2025 Seena et al. This is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Seena, T.X., Elizabeth Kurian, Lali, F.A., Rojan, P.M., Bindu, K.A., Aziz Zarina and George Sherin, K. 2025. High-resolution melting analysis of Phospholipase C gamma 1 (PLCG1) gene in Vechur and Kasargod cattle of Kerala.
J. Vet. Anim. Sci. 56 (3):424-428