Volume: 56 Issue: 4
Year: 2025, Page: 687-691, Doi: https://doi.org/10.51966/jvas.2025.56.4.687-691
Received: Sept. 19, 2025 Accepted: Oct. 10, 2025 Published: Dec. 31, 2025
The incidence of Blastocystis sp. in poultry from Puducherry, India was recorded in both backyard and broiler chicken. A total of 200 intact whole intestines of both backyard (50 nos.) and broiler chicken (150 nos.) were procured from local chicken outlets as well as from the nearby villages covering 13 places. The area-wise percentage of incidence of Blastocystis was recorded. The study involved qualitative examination and different staining techniques to detect the different forms of Blastocystis viz., Giemsa staining, Gram’s staining and modified acid-fast staining. Out of 200 intestinal samples comprising of both broilers and desi birds (150:50), 135 intestines were found positive for Blastocystis (broiler 112 nos and desi 23 nos), representing an overall infection 67.5 per cent (135/200) in chicken (74.66% infection in broiler and 46% infection desi birds.) Among the different staining techniques used, the vacuolar form of Blastocystis was found to be predominant compared to granular form. Modified acid fast staining was found more sensitive followed by Giemsa stain.
Keywords: Blastocystis, Broiler and Desi birds, Incidence, Staining, Puducherry, India
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© 2025 Shiyamala et al. This is an open access article distributed under the terms of the Creative Commons Attribution 4.0
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Shiyamala, S., Das, S.S., Sreekrishnan, R. and Mathivathani, C. 2025. Incidence of Blastocystis sp. in chicken from Puducherry, India. J. Vet. Anim. Sci. 56 (4): 687-691