Volume: 56 Issue: 4
Year: 2025, Page: 671-675, Doi: https://doi.org/10.51966/jvas.2025.56.4.671-675
Received: Sept. 11, 2025 Accepted: Oct. 3, 2025 Published: Dec. 31, 2025
Heat stress is a critical environmental factor influencing cellular physiology and has implications for livestock cell biology and regenerative research. This study evaluated the effects of acute (2 h) and chronic (24 h) heat exposure on proliferation and morphology of bovine adipose-derived stromal/stem cells (bADSCs). Bovine ADSCs were isolated from subcutaneous adipose tissue (~5 g) by collagenase digestion and in vitro cultured in DMEM supplemented with 15 per cent FBS and one per cent antibiotic–antimycotic. Cells attached within 24–48 h, adopted a spindle-shaped fibroblastic morphology by days 3–5, and reached 80–90 per cent confluence by 13–14 days; morphology and growth remained stable through passages 6–8. For heat stress experiments, third-passage cells were seeded (5 × 104 cells/ well) and exposed to 39°C or 41°C (controls at 37°C). Proliferation was assessed by Trypan Blue dye exclusion and expressed as ×103 cells/mL. Under acute exposure, proliferation at 39°C (68.0 ± 3.60) exceeded that at 37°C (55.0 ± 1.00) and 41°C (54.0 ± 6.92), but differences were not statistically significant (p = 0.13). After 24 h, proliferation modestly increased across groups with values of 72.0 ± 5.20 (39°C), 68.0 ± 4.46 (37°C) and 62.0 ± 6.08 (41°C) (p = 0.45). Overall, 39°C produced the highest proliferation at both time points, though effects did not reach significance. These findings suggest that mild hyperthermia (39°C) may transiently stimulate bADSC metabolic activity and proliferation, possibly via. pro-survival signalling and induction of heat shock responses, whereas higher thermal load (41°C) tends toward neutral or slightly adverse effects.
Keywords: Bovine adipose-derived stromal cells, heat stress, cell morphology, proliferation, Trypan Blue
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© 2025 Harippriya et al. This is an open access article distributed under the terms of the Creative Commons Attribution 4.0
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Harippriya, K., Pratheesh, M.D., Karthiayini, K., Raji, K., Sathu, T. and Varghese, R. 2025. Influence of heat stress on the proliferation of bovine adipose-derived stromal cells. J. Vet. Anim. Sci. 56 (4): 671-675