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Journal of Veterinary and Animal Sciences

Volume: 57 Issue: 1

  • Open Access
  • Research Article

Physico-chemical characteristics of commercially available and cold slaughtered broiler chicken meat

A. Balakrishnan1*, V.N. Vasudevan 1, A. Irshad 1, S. Sasi1, R.Uma2 and S.N. Nair3

1Department of Livestock Products Technology, 2Department of Veterinary Biochemistry, College of Veterinary and Animal Sciences, Mannuthy, Thrissur, Kerala, 3Department of Veterinary Pharmacology and Toxicology, College of Veterinary and Animal Sciences , Pookode, Kerala Veterinary and Animal Sciences University, Wayanad, Kerala, India

Year: 2026, Page: 104-111, Doi: https://doi.org/10.51966/jvas.2026.57.1.104-111

Received: Sept. 12, 2025 Accepted: Nov. 3, 2025 Published: March 31, 2026

Abstract

In the current study, leg muscle samples of commercially available (CA) broiler chicken slaughtered traditionally by neck severance and cold slaughtered (CS) broiler chicken carcasses were compared for their physico-chemical characteristics. Sample harvesting was carried out at 0-2, 2-4, 4-6 and 6-8 h post mortem. The pH revealed significantly (p<0.01) lower values in CS leg samples compared to CA leg samples at all times of sample collection except at 6-8 h. Significantly lower pH values were recorded in samples collected at increasing post mortem time intervals with mean pH values shifting from 6.3±0.05 at 0-2 h to 5.78±0.05 at 6-8 h in CA samples. Water holding capacity (WHC) decreased significantly as time of sample collection post mortem increased with significantly lower WHC in CS group (48±0.894 % at 6-8 h), and thiobarbituric acid reactive substances (TBARS) values increased significantly as time of sample collection post mortem increased with mean values of 0.26±0.01 and 0.5±0.02, in CA and CS samples respectively at 6-8 h. The L* values were higher in CA samples whereas a* and b*colour values were higher in CS samples. As time of sample collection post mortem increased, the mean a* values decreased from 3.9±0.33 at 0-2 h to 2.54±0.35 at 6-8 h in CA samples and from 11.17±0.76 at 0-2 h to 9.48±0.84 at 6-8 h in CS samples. In both CA and CS samples, L* values increased significantly as time of sample collection post mortem increased. Haem iron, haemoglobin and myoglobin contents of muscle samples were significantly higher in CS group, but as the time of sample collection post mortem increased the difference became less significant.

Keywords: Broiler chicken meat, cold slaughter, commercial, post mortem, meat pigments

References

Dead chicken kept for sale in Kerala under code name ‘M’. (2023, January 16). Mathrubhumi News. https://english.mathrubhumi.com/news/kerala/dead-chicken-still-sold-in-kerala-under-code-name-m--98a2fbe4

Abbas, N., Ali, A., Kumari, S., Iqbal, A., Husain, A., Saeed, T., Al-Ballam, Z. A., Ahmed, N., El-Seedi, H. R., & Musharraf, S. G. (2020). Untargeted metabolomics differentiation between poultry samples slaughtered with and without detaching spinal cord. Arabian Journal of Chemistry, 13(12), 9081–9089.

Abhishek, R. (2012, June 6). Roadside food stalls in Hyderabad using dead chicken meat. The Times of India. https://timesofindia.indiatimes.com/is-that-dead-chicken-on-your-plate/articleshow/13109029

Addeen, A., Benjakul, S., Wattanachant, S., & Maqsood, S. (2014). Effect of Islamic slaughtering on chemical composition and post-mortem quality changes of broiler chicken meat. International Food Research Journal, 21, 897–907.

Ahmad, T. A., Kumar, Y. K., Gaikwad, N. G., & Rai, D. R. (2015). Correlation between some quality parameters and bleeding status of poultry meat. Asian Journal of Dairy and Food Research, 34(4), 332–334.

Alvarado, C. Z., Richards, M. P., O’Keefe, S. F., & Wang, H. (2007). The effect of blood removal on oxidation and shelf life of broiler breast meat. Poultry Science, 86, 156–161.

Belore, B. M., Maheswarappa, N. B., Kulkarni, V. V., Banerjee, R., Hazarika, P., Dasoju, S., Mishra, B. P., & Govindaiah, P. M. (2023). Biomarker discovery and authentication of cold-slaughtered chicken through classical analytical procedures and mass spectrometry-based proteomic approaches. British Poultry Science, 64, 605–613.

Benjakul, S., & Bauer, F. (2001). Biochemical and physicochemical changes in catfish (Siluru sglanis Linne) muscle as influenced by different freeze–thaw cycles. Food Chemistry, 72, 207–217.

Bhawana, I., Malik, A., Raposo, A., Singh, S., Yadav, S., Zandonadi, R. P., Lho, L. H., Han, H., & Thakur, N. (2023). Physico-chemical, sensory, and microbiological quality of raw chicken meat: An exploratory study in Hisar city of Haryana, India. Frontiers in Nutrition, 10, Article 1184005.

Boulianne, M., & King, A. J. (1998). Meat color and biochemical characteristics of unacceptable dark-colored broiler chicken carcasses. Journal of Food Science, 63, 759–762.

Cao, M., Han, Q. A., Zhang, J., Zhang, R., Wang, J., Gu, W., Kang, W., Lian, K., & Ai, L. (2020). An untargeted and pseudotargeted metabolomic combination approach to identify differential markers to distinguish live from dead pork meat by liquid chromatography–mass spectrometry. Journal of Chromatography A, 1610, 460–553.

Crystal, B. B., Devine, C. E., & Newton, K. G. (1981). Residual blood in lamb muscles. Meat Science, 5, 339–345.

Department of Animal Husbandry and Dairying. (2024). Basic animal husbandry statistics. Government of India.

Devatkal, S. K., Naveena, B. M., & Kotaiah, T. (2019). Quality, composition, and consumer evaluation of meat from slow-growing broilers relative to commercial broilers. Poultry Science, 98(11), 6177–6186.

Food and Agriculture Organization of the United Nations. (2024). The state of food and agriculture 2024: Value-driven transformation of agrifood systems. FAO.

Goyal, M. M., & Basak, A. (2009). Estimation of plasma haemoglobin by a modified kinetic method using o-tolidine. Indian Journal of Clinical Biochemistry, 24(1), 36–41.

Hamm, R. (1961). Biochemistry of meat hydration. In Advances in food research (Vol. 10, pp. 355–463). Academic Press.

Krzywicki, K. (1979). Assessment of relative content of myoglobin, oxymyoglobin and metmyoglobin at the surface of beef. Meat Science, 3(1), 1–10.

Lawrie, R. A. (1991). Meat science (5th ed.). Pergamon Press.

Lee, E. J., & Shin, H. S. (2019). Development of a freshness indicator for monitoring the quality of beef during storage. Food Science and Biotechnology, 28(6), 1899–1906.

MacDougall, D. B., & Rhodes, D. N. (1972). Characteristics of the appearance of meat: III. Studies on the colour of meat from young bulls. Journal of the Science of Food and Agriculture, 23(5), 637–647.

O’Brien, P. J., Shen, H., McCutcheon, L. J., O’Grady, M., Byrne, P. J., Ferguson, H. W., Mirsalimi, M. S., Julian, R. J., Sargeant, J. M., Tremblay, R. R., & Blackwell, T. E. (1992). Rapid, simple and sensitive microassay for skeletal and cardiac muscle myoglobin and hemoglobin: Use in various animals indicates functional role of myohemoproteins. Molecular and Cellular Biochemistry, 112(1), 45–52.

O’Halloran, G. R., Troy, D. J., & Buckley, D. J. (1997). The relationship between early post-mortem pH and the tenderisation of beef muscles. Meat Science, 45, 239–251.

Oellingrath, I. M., Iversen, A., & Skrede, G. (1990). Quantitative determination of myoglobin and haemoglobin in beef by high-performance liquid chromatography. Meat Science, 28, 313–320.

Pavan, K. B. (2020, November 20). Adulterated meat being served in restaurants; authorities seize tonnes of mutton. The Hans India.

Petracci, M., & Fletcher, D. L. (2002). Broiler skin and meat color changes during storage. Poultry Science, 81(10), 1589–1597.

Qiao, M., Fletcher, D. L., Smith, D. P., & Northcutt, J. K. (2001). The effect of broiler breast meat color on pH, moisture, water-holding capacity, and emulsification capacity. Poultry Science, 80(5), 676–680.

Quawsar, A. M., Afrin, F., Begum, M. I., Haider, M. G., Rauf, S. M., & Golbar, H. M. (2024). Differentiation of meat from dead and slaughtered animals based on hemoglobin content. Journal of Biosciences, 32(1), 31–39.

Rahman, H. U. U., Sahar, A., Khan, M. I., & Jamil, A. (2019). Discerning microbial and quality attributes of differently slaughtered and dead poultry [n1] meat. Journal of Food Safety, 39(3), e12622.

Richards, M. P., & Hultin, H. O. (2002). Contributions of blood and blood components to lipid oxidation in fish muscle. Journal of Agricultural and Food Chemistry, 50(3), 555–564.

Sabikun, N., Bakhsh, A., Ismail, I., Hwang, Y. H., Rahman, M. S., & Joo, S. T. (2019). Changes in physicochemical characteristics and oxidative stability of pre- and post-rigor frozen chicken muscles during cold storage. Journal of Food Science and Technology, 56(11), 4809–4816.

Sasidharan, V., Vasudevan, V. N., Sunil, B., Sathu, T., Ramnath, V., Sankar, K. A., & Yazhinidevi, R. (2022). Evaluation of colour and bleeding efficiency of imperfectly bled, scientifically slaughtered and cold-slaughtered beef carcasses. Journal of Veterinary and Animal Sciences, 53, 385–391.

Saud, S., Li, G., Kong, H., Khan, M. I., Qiang, Z., Sun, Y., Liu, W., Ding, C., Xiao, H., Wang, Y., & Li, H. (2019). Identification of chicken meat quality via rapid array isoelectric focusing with extraction of hemoglobin and myoglobin in meat samples. Journal of Chromatography B, 1128, 121–790.

Schreurs, F. J. G. (2000). Post-mortem changes in chicken muscle. World’s Poultry Science Journal, 56(4), 319–346.

Si, R., Zhang, X., Yao, Y., Zhang, S., & Wang, H. (2021). Unpacking the myth between increased government initiatives and reduced selling of dead livestock in China: Exploring hidden dangers of zoonotic diseases. One Health, 13, Article 100344.

Sidwick, K. L., Johnson, A. E., Adam, C. D., Pereira, L., & Thompson, D. F. (2017). Use of liquid chromatography quadrupole time-of-flight mass spectrometry and metabonomic profiling to differentiate between normally slaughtered and dead-on-arrival poultry meat. Analytical Chemistry, 89(22), 12131–12136.

Sohaib, M., Zafar, M. S., Arshad, M. S., Nauman, K., & Malhi, I. Y. (2020). Evaluation of quality and safety attributes of slaughtered versus dead chicken birds’ meat. Brazilian Journal of Poultry Science, 22, 1–10.

Sujiwo, J., Kim, D., & Jang, A. (2018). Relation among quality traits of chicken breast meat during cold storage: Correlations between freshness traits and torrymeter values. Poultry Science, 97(8), 2887–2894.

Warriss, P. D. (1979). The extraction of haem pigments from fresh meat. International Journal of Food Science & Technology, 14(1), 75–80.

Witte, V. C., Krause, J. F., & Bailey, M. E. (1970). A new extraction method for determining 2-thiobarbituric acid values of pork and beef during storage. Journal of Food Science, 35, 582–585.

 


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Cite this article

Balakrishnan., A., Vasudevan V.N., Irshad A., Sasi, S., Uma, R. & Nair, S.N. (2026). Physicochemical characteristics of commercially available and cold slaughtered broiler chicken meat, Journal of Veterinary and Animal Sciences, 57(1), 104-111 https://doi.org/10.51966/jvas.2026.57.1.104-111

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