Volume: 56 Issue: 3
Year: 2025, Page: 457-463, Doi: https://doi.org/10.51966/jvas.2025.56.3.457-463
Received: March 3, 2025 Accepted: May 12, 2025 Published: Sept. 30, 2025
Brucellosis is an economically important zoonotic disease of dairy industry. This study was designed to assess individual animal and herd level seroprevalence of bovine brucellosis in dairy herds located in Southwestern Punjab, India. A total of 898 bovine [cattle (n= 623); buffaloes (n= 275)] serum samples from six districts [Bathinda (n= 769), Barnala (n= 100), Muktsar (n= 22), Mansa (n= 03), Faridkot (n= 03) and Moga (n= 01)] of Southwestern Punjab were received for diagnosis of brucellosis. The samples were belonging to 260 dairy herds and animals were suffering from reproductive disorders such as abortion, infertility, repeat breeding, hygroma etc. Initially, the Rose Bengal plate agglutination test (RBPT) was performed to screen serum samples for qualitative detection of anti-Brucella antibodies. In RBPT, 31.18 per cent (280/898) of serum samples were positive for brucellosis. Among the 260 herds, 98 (37.69%) herds were positive for the disease. The RBPT-positive samples were further processed for quantitative detection of antibody titre using standard plate agglutination test (STAT). In STAT, 72.50 per cent (203/280) of RBPT-positive samples were positive. The highest prevalence was reported in Mansa (66.67%), followed by Bathinda (32.89%), Barnala (23%), and Muktsar (9.1%). None of the samples from Faridkot and Moga districts tested positive. Three host factors, viz, species, sex, and age, were included in the analysis of disease status. There was no statistical variation in the disease prevalence among cattle and buffaloes, and males and females. However, the prevalence was higher among animals aged more than one year (30.21-34.24%) than those aged less than one year (22.22%). Further studies need to be conducted with large sample size to determine the true burden of disease in Punjab, especially in the Southwestern region.
Keywords: Bovine Brucellosis, Punjab, Seroprevalence, Zoonosis
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© 2025 Yadav and Singh. This is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Yadav, J.P. and Singh, M. 2025. Seroprevalence of brucellosis among bovine populations of Southwestern Punjab, India.
J. Vet. Anim. Sci. 56 (3):457-463