Volume: 48 Issue: 2
Year: 2017, Page: 6-12,
Received: May 4, 2017 Accepted: July 8, 2017 Published: Dec. 31, 2017
Haemonchus contortus is one of the most important parasites causing reduced production in livestock throughout the tropics and in most other countries. The present study has been undertaken to screen and evaluate a commonly used plant of Tamil Nadu namely, Aristolochia indica for its anthelmintic activity against H. contortus using in vitro test. Four different types of extracts viz aqueous, ethanol, chloroform and acetone were prepared and used for larval paralysis assay. The results revealed that all the extracts produced significant inhibition of the larval motility in vitro. At the end of one hour, the results showed that ethanolic extract (73.88 – 91.66%) was most effective, followed by aqueous (62.5–91.50%) and chloroform extract (42.42- 91.66%). The larval paralysis produced by all the extracts at the highest concentrations tested was below that of the positive controls, ivermectin (85-100%) and levamisole (90-100%). Results indicated that the ethanolic, aqueous and chloroform extracts of A. indica contained certain compounds active against L3 larva, potent enough to inhibit its normal motility. The larval paralytic action produced within a short duration of one hour also reflects that the active moieties were well absorbed through the larval cuticle. LPA results of this study showed that the L3 paralytic activity was consistently above 90% in three of the A. indica extracts, except its acetone extract with a lower activity, which shows a strong potential for future use as anthelmintic for replacing synthetic compounds with resistance.
Keywords: Anthelmintic activity, Aristolochia indica extracts, Haemonchus contortus, Larval paralysis assay.
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