Volume: 53 Issue: 2
Year: 2022, Page: 195-200, Doi: https://doi.org/10.51966/jvas.2022.53.2.195-200
Received: Dec. 21, 2020 Accepted: Oct. 12, 2021 Published: June 30, 2022
The aim of this study was to evaluate the astrocytic reaction in infected brains of dogs in that had been afflicted with the furious and paralytic form of rabies. Brain samples from 23 dogs that were positive for rabies were collected along with six brain samples that were negative for rabies. GFAP (Glial fibrillary acidic protein), specific astrocytic marker was used to differentially analyse the immunoreactivity of astrocytes to GFAP in specific brain regions in two the clinical forms of rabies viz, the furious and paralytic forms. The study showed the extent of astrogliosis in specific regions of brain in the two forms of rabies and also the intensity of GFAP expression. Region wise astrogliosis, was more diffuse in the brains of animals afflicted with the furious form of rabies but were more localized in the brains of animals with the paralytic form, specifically into the white matter of cerebellum
Keywords: Rabies, Astrocytes, GFAP
Brat, D.J. 2018. Normal brain histopathology. Practical surgical neuropathology: A diagnostic approach (2nd Ed.). Elsevier. 21-22 pp.
Delcambre, G.H., Liu, J., Herrington, J.M., Vallario, K. and Long, M.T. 2016. Immunohistochemistry for the detection of neural and inflammatory cells in equine brain tissue. Peer J. 4: 1601-1605.
Headley, S.A., Soares, I.C. and Graça, D.L. 2001. Glial fibrillary acidic protein (GFAP)-immunoreactive astrocytes in dogs infected with canine distemper virus. J. Comp. Pathol. 125: 90-97.
Hol, E.M. and Pekny, M. 2015. Glial fibrillary acidic protein (GFAP) and the astrocyte intermediate filament system in diseases of the central nervous system. Curr. Opin. Cell Biol. 32: 121-130.
Kojima, D., Park, C.H., Satoh, Y., Inoue, S., Noguchi, A. and Oyamad, T. 2009. Pathology of the spinal cord of C57BL/6J mice infected with rabies virus (CVS-11 strain). J. Vet. Med. Sci. 71: 319-324.
Machado, G.F. and Alessi, A.C. 1997. Glial fibrillary acidic protein (GFAP) immunoreactive astrocytes in the CNS of normal and rabies-infected adult cattle. I. Hippocampus and dentate gyrus. Braz. J. Vet. Res. Anim. Sci. 34: 332-335.
Soung, A. and Klein, R.S. 2018. Viral encephalitis and neurologic diseases: focus on astrocytes. Trend. Mol. Med. 24: 950-962.
Venugopal, A.K., Ghantasala, S.S.K., Selvan, L.D.N., Mahadevan, A., Renuse, S., Kumar, P., Pawar, H., Sahasrabhuddhe, N.A., Suja, M.S., Ramachandra, Y.L. and Prasad, T.S.K. 2013. Quantitative proteomics for identifying biomarkers for Rabies. Clin. Proteom. 10: 1-13.
Tepsumethanon, V., Likitsuntonwong, W., Thorner, P.S. and Shuangshoti, S. 2016. Dogs that develop rabies post-vaccination usually manifest the paralytic subtype. Prev. Vet. Med. 131: 64-66.
Zhan, J.S., Gao, K., Chai, R.C., Jia, X.H., Luo, D.P., Ge, G., Jiang, Y.W., Fung, Y.W.W., Li, L. and Yu, A.C.H. 2017. Astrocytes in migration. Neurochem. Res. 42: 272-282
© 2022 Shruthi et al. This is an open access article distributed under the terms of the Creative
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Shruthi, A., Prasanna, K. S., Sachin and Ajith, J. G. 2022. Astrocytic reaction in furious and paralytic forms of rabies with reference to GFAP expression in rabies positive dog brain samples. J. Vet. Anim. Sci. 53(2): 195-200
DOI: https://doi.org/10.51966/jvas.2022.53.2.195-200