Volume: 54 Issue: 3
Year: 2023, Page: 763-771, Doi: https://doi.org/10.51966/jvas.2023.54.3.763-771
Received: Feb. 23, 2023 Accepted: April 11, 2023 Published: Sept. 30, 2023
This study was undertaken to assess the probiotic and antioxidant potential of five indigenous cultures of lactic acid bacteria viz, Lactobacillus acidophilus, Lactiplantibacillus plantarum, Lacticaseibacillus rhamnosus, Lactobacillus bulgaricus and Streptococcus thermophilus. The probiotic properties in terms of acid and bile tolerance, cell surface hydrophobicity, autoaggregation, coaggregation and bile salt hydrolase activity were assessed in vitro. The DPPH assay indicated highest antioxidant activity of 45.33 per cent for Streptococcus thermophilus. All five cultures exhibited significant antioxidant potential.Lacticaseibacillus rhamnosus, Lactobacillus acidophilus and Lactiplantibacillus plantarum showed higher probiotic potential in terms of acid and bile tolerance, cell surface hydrophobicity, autoaggregation and coaggregation. None of the cultures exhibited bile salt hydrolase activity.
Keywords: Lactic acid bacteria, probiotic, antioxidant
Abubakr, M. A., Hassan, Z., Imdakim, M. M. A. and Sharifah, N. R. S. A. 2012. Antioxidant activity of lactic acid bacteria (LAB) fermented skim milk as determined by 1, 1-diphenyl-2-picrylhydrazyl (DPPH) and ferrous chelating activity (FCA). Afr. J.Microbiol. Res.6(34): 6358-6364.
Anandharaj, M., Sivasankari, B., Santhanakaruppu, R., Manimaran, M., Rani, R. P. and Sivakumar, S. 2015. Determining the probiotic potential of cholesterol-reducing Lactobacillus and Weissella strains isolated from gherkins (fermented cucumber) and south Indian fermented Koozh. Res. Microbiol.166(5): 428-439.
Balamurugan, R., Chandragunasekaran, A. S., Chellappan, G., Rajaram, K., Ramamoorthi, G. and Ramakrishna, B. S. 2014. Probiotic potential of lactic acid bacteria present in home made curd in southern India. Indian J.Med.Res. 140(3): 345-355.
Biswas, S., Keightley, A. and Biswas, I. 2019. Characterization of a stress tolerance‐defective mutant of Lactobacillus rhamnosus LRB. Mol. Oral Microbiol. 34(4): 153-167.
Collado, M. C., Meriluoto, J. and Salminen, S. 2008. Adhesion and aggregation properties of probiotic and pathogen strains. Eur. Food Res. Technol. 226: 1065-1073.
Ekmekci, H., Aslim, B. and Ozturk, S. 2009. Characterization of vaginal lactobacilli coaggregation ability with Escherichia coli. Microbiol. Immunol. 53(2): 59-65.
Fijan, S. 2014. Microorganisms with claimed probiotic properties: an overview of recent literature. Int. J. Environ. Res. Public Hlth. 11(5): 4745-4767.
Goldin, B. R. 1998. Health benefits of probiotics. Br. J. Nutr. 80(S2): S203-S207.
Gotcheva, V., Hristozova, E., Hristozova, T., Guo, M., Roshkova, Z. and Angelov, A. 2002. Assessment of potential probiotic properties of lactic acid bacteria and yeast strains. Food Biotech. 16(3): 211-225.
Hamon, E., Horvatovich, P., Izquierdo, E., Bringel, F., Marchioni, E., Aoudé-Werner, D. and Ennahar, S. 2011. Comparative proteomic analysis of Lactobacillus plantarum for the identification of key proteins in bile tolerance. BMC Microbiol. 11: 1-11.
Harty, D. W. S., Patrikakis, M. and Knox, K. W. 1993. Identification of Lactobacillus strains isolated from patients with infective endocarditis and comparison of their surface-associated properties with those of other strains of the same species. Microbial Ecol. Hlth Dis. 6(4): 191-201.
Kos, B., Uskovic, J.S., Vukovic, S., Impraga, M.S., Frece, J. and Matosic, S. 2003. Adhesion and aggregation ability of probiotic strain Lactobacillus acidophilus M92. J. Appl. Microbiol. 94: 981–987.
Lee, H., Yoon, H. and Ji, Y. 2011. Functional properties of Lactobacillus strains isolated from kimchi. Int. J. Food Microbiol.145: 155–161.
Lee, M., Hong, G. E., Zhang, H., Yang, C. Y., Han, K. H., Mandal, P. K. and Lee, C. H. 2015. Production of the isoflavone aglycone and antioxidant activities in black soymilk using fermentation with Streptococcus thermophilus S10. Food Sci. Biotech. 24: 537-544.
Leroy, F. and De Vuyst, L. 2004. Lactic acid bacteria as functional starter cultures for the food fermentation industry. Trends Food Sci. Technol. 15(2): 67-78.
Ogunyemi, O., Gyebi, G., Shaibu, R., Fabusiwa, M. and Olaiya, C. 2021. Antioxidant, Nutritional, and Physicochemical Quality of Yoghurt Produced from a Milk-Based Fermentation Mix Enhanced with Food Spices. Croatian J. Food Sci. Technol. 13(2): 201-209.
Polak-Berecka, M., Waśko, A., Paduch, R., Skrzypek, T. and Sroka-Bartnicka, A. 2014. The effect of cell surface components on adhesion ability of Lactobacillus rhamnosus. Antonie Van Leeuwenhoek. 106: 751-762.
Pundir, R. K., Kashyap, S. R. N. and Kaur, A. 2013. Probiotic potential of lactic acid bacteria isolated from food samples: an in vitro study. J. Appl. Pharmaceutical Sci. 3(3): 085-093.
Reid, G., Cuperus, P. L., Bruce, A. W., Van der Mei, H. C., Tomeczek, L., Khoury, A. H. and Busscher, H. J. 1992. Comparison of contact angles and adhesion to hexadecane of urogenital, dairy, and poultry lactobacilli: effect of serial culture passages. Appl. Environ. Microbiol. 58(5): 1549-1553.
Sabir, F., Beyatli, Y., Cokmus, C. andOnal‐Darilmaz, D. 2010. Assessment of potential probiotic properties of Lactobacillus spp., Lactococcus spp., and Pediococcus spp. strains isolated from kefir. J. Food Sci. 75(9): M568-M573.
Sharma, A., Lavania, M., Singh, R. and Lal, B. 2021. Identification and probiotic potential of lactic acid bacteria from camel milk. Saudi J. Biol. Sci. 28(3): 1622-1632.
Zhang, Y., Hu, P., Lou, L., Zhan, J., Fan, M., Li, D. and Liao, Q. 2017. Antioxidant activities of lactic acid bacteria for quality improvement of fermented sausage. J. Food Sci. 82(12): 2960-2967.
© 2023 Anija et al. This is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Anija, S.M., Sreya U., Aparna, S.V., Beena, A.K., Lijimol, J. and Beena, R.L. 2023. Comparative evaluation of the probiotic and antioxidant potential of indigenous Lactic Acid Bacteria. J. Vet. Anim. Sci. 54(3):763-771
DOI: https://doi.org/10.51966/jvas.2023.54.3.763-771