Volume: 54 Issue: 3
Year: 2023, Page: 855-858, Doi: https://doi.org/10.51966/jvas.2023.54.3.855-858
Received: April 4, 2023 Accepted: June 30, 2023 Published: Sept. 30, 2023
Feline dermatophytosis is a commonly encountered fungal skin disease in cats. Skin scrapings from cats with varying degrees of clinical signs were collected and subjected to microscopic and cultural examination. The clinical samples were stained with three different stains, lactophenol cotton blue (LPCB), Chicago sky blue (CSB) and Calcofluor white (CW) and were subjected to microscopic examination for fungal spores. The results of this microscopic examination were analysed and compared with growth of dermatophytes in Dermatophyte test medium (DTM). Based on cultural isolation, dermatophytosis was confirmed in 36 out of 82 cases tested. Comparison of staining techniques revealed that CW staining had better sensitivity (97.22 per cent), specificity (65.22 per cent) and overall agreement with cultures in DTM. Occurrence was found to be higher in kittens less than six months of age and long-haired breeds.
Keywords: Feline dermatophytosis, lactophenol cotton blue, chicago sky blue, calcofluor white, dermatophyte morphology
Ashwini, A., Chirayath, D., Pillai, U.N., Rajan, S.K. and Radhika, R. 2020. Zoonotic dermatophytosis by micro-sporum canis in cats. J. Vet. Anim. Sci. 51: 94-96
Cafarchia, C., Romito, D., Sasanelli, M., Lia, R., Capelli, G. and Otranto, D. 2004. The epidemiology of canine and feline dermatophytoses in southern Italy. Mycoses. 47: 508-513.
Dass, S.M., Vinayaraj, E., Pavavni, K., Pallam, A. and Rao, M.S. 2015. Comparison of KOH, calcofluor white and fungal culture an for diagnosing fungal onychomycosis in urban teaching hospital, Hyderabad. Indian J. Microbiol. Res. 2: 148-53.
Debnath, C., Mitra, T., Kumar, A. and Samanta, I. 2016. Detection of dermatophytes in healthy companion dogs and cats in eastern India. Iran. J. Vet. Res. 17: 20.
Gross, T.L., Ihrke, P.J., Walder, E.J. and Affolter, V.K. 2008. Skin diseases of the dog and cat: clinical and histopathologic diagnosis. (2nd Ed.). John Wiley & Sons, 410p.
Moriello, K.A. 2004. Treatment of dermatophytosis in dogs and cats: Review of published studies. Vet. Dermatol. 15: 99-107.
Moriello, K.A. and Newbury, S. 2006. Recommendations for the management and treatment of dermatophytosis in animal shelters. Veterinary Clinics: Small Animal Practice. 36: 89-114.
Prakash, V.A., Rathish, R.L., Deepa, P.M., Bipin, K.C. and John, L. 2022. Host and environmental risk factors of canine dermatophytosis. Pharm. Innov. 11: 936 939.
Prakash, V.A., Rathish, R.L., Deepa P.M., Bipin K.C. and John, L. 2023. Comparative efficacy of three stains for the diagnosis of canine dermatophytosis. Indian Vet. J. 100: 27 – 31
Rich, P., Harkless, L.B. and Atillasoy, E.S. 2003. Dermatophyte test medium culture for evaluating toenail infections in patients with diabetes. Diabetes Care 26: 1480 1484.
Robert, R. and Pihet, M. 2008. Conventional methods for the diagnosis of dermatophytosis. Mycopathologia 166: 295-306.
Tarra, M., Davis, K.J., Vinodkumar, K., Vijayakumar, K. and Menon, V.K. 2022. Occurrence of dermatophytosis in dogs from Thrissur, Kerala. J. Vet. Anim. Sci. 53: 322-327.
Tunsagool, P., Ploypetch, S., Jaresitthikunchai, J., Roytrakul, S., Choowongkomon, K. and Rattanasrisomporn, J. 2021. Efficacy of cyclic lipopeptides obtained from bacillus subtilis to inhibit the growth of microsporum canis isolated from cats. Heliyon 7: e07980.
© 2023 Sheetal et al. This is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Sheetal, M.S., Rathish, R.L., Janus, A., Sindhu, O.K., Cyril, T. and Deepa, P.M. 2023. Comparison of three staining techniques for rapid diagnosis of feline dermatophytosis. J. Vet. Anim. Sci. 54(3):855-858 DOI: https://doi.org/10.51966/jvas.2023.54.3.855-858