Volume: 51 Issue: 1
Year: 2020, Page: 25-30,
Received: June 18, 2019 Accepted: July 30, 2019 Published: Jan. 1, 2020
Rotaviral enteritis is a common condition observed in farm animals especially piglets and calves. Though the presence of porcine rotaviruses (PRV) have been reported in pigs in Kerala, no study has been conducted to characterize them. This paper reports the finding of a study conducted to detect and characterize PRV based on VP6 gene. A total of 87 samples collected from cases of piglet diarrhoea were subjected to VP6 gene based reverse transcriptase polymerase chain reaction (RT-PCR) and five (5.74 per cent) was found to be positive. All the positive samples were from Palakkad district. On analysis of the nucleotide sequence it was observed that the viruses belonged to inner capsid type I5 and I14 indicating diversity in the PRV prevalent in Kerala.
Keywords: Rotavirus, pigs, reverse transcriptase polymerase chain reaction, VP6 gene
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© 2020 Logeshwaran et al. This is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Logeshwaran, G., Ravishankar, C., Nandhakumar, D., Sebastian, S. R., Sumod, K., Jayakrishnan, T. R., Ravindran, R. and John, K. 2020. Detection and VP6 gene based molecular characterisation of rotaviruses of pigs in Kerala. J. Vet. Anim. Sci. 51(1): 25-30.