Volume: 54 Issue: 2
Year: 2023, Page: 510-515, Doi: https://doi.org/10.51966/jvas.2023.54.2.510-515
Received: Nov. 14, 2022 Accepted: Feb. 13, 2023 Published: June 30, 2023
Indoxacarb is a novel organophosphate substitute and is considered a “lower risk” pesticide in agriculture. However, in humans and animals, indoxacarb exposure had been linked to substantial health problems. This study was carried out to investigate the acute oral toxicity and histopathological changes of indoxacarb in female Wistar albino rats. The acute oral toxicity of indoxacarb was performed as per the OECD guidelines No. 425, (Acute Oral Toxicity - Up-anddown procedure). The LD50 of indoxacarb was estimated using AOT425StatPgm (version: 1.0) statistical program and found to be 1098 mg/kg. Hence the pesticide is categorized as Type II class of pesticide according to the WHO standards. All the animals dosed at 2000 mg/kg died within 12 h exhibiting clinical signs of intermittent episodes of tremors, incoordination, respiratory distress and open-mouth breathing. Histopathological examination of indoxacarb treated rats at 2000mg/ kg degeneration of hepatocytes and necrosis in haematoxylin and eosin staining.
Keywords: Indoxacarb, oral LD50, wistar rats, acute oral toxicity, hepatotoxicity
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© 2023 Raj et al. This is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Raj, A., Nair, N.S., Nisha, A.R., Sujith, S., Divya C. and Shankar R. Determination of the median lethal dose of indoxacarb pesticide in Wistar rat. J. Vet. Anim. Sci. 54(2):510-515
DOI: https://doi.org/10.51966/jvas.2023.54.2.510-515