Volume: 54 Issue: 2
Year: 2023, Page: 443-449, Doi: https://doi.org/10.51966/jvas.2023.54.2.443-449
Received: Nov. 10, 2022 Accepted: Feb. 14, 2023 Published: June 30, 2023
An experiment was conducted in the Department of Poultry Science, College of Veterinary and Animal Sciences, Mannuthy to find out the effect of probiotics and rice on ileal microbial count and intestinal morphometry in broiler chicken for a period of 42 days. A total of 144, day-old Ven Cobb 430 Y broiler chicks were allotted randomly to six dietary treatment groups with three replicates of eight birds each. The treatment groups were T1-standard broiler ration (SBR1), T2- SBR1 with 0.05 per cent probiotic mixture, T3- SBR1 with 0.1 per cent probiotic mixture, T4- 50 per cent of maize from SBR1 replaced by rice (SBR2), T5- SBR2 with 0.05 per cent probiotic mixture and T6- SBR2 with 0.1 per cent probiotic mixture. The dietary inclusion of rice did not affect the E. coli count and ileal pH. However the coliform count was significantly (p<0.01) lower in group fed rice based diet supplemented with 0.1 per cent probiotics and the E. faecalis count was significantly (p<0.01) higher in rice fed group without probiotic supplementation than corn fed group without probiotic supplementation. The probiotic supplementation at 0.1 per cent level significantly (p<0.01) reduced the E. coli and coliform counts in both rice and corn based diets. The E. faecalis count and ileal pH was significantly (p<0.01) reduced by probiotics at 0.05 and 0.1 per cent levels in corn based as well as rice based diets. The dietary inclusion of rice in broiler diet significantly increased (p<0.01) the ileal villi height in the non-supplemented group. The ileal VH:CD ratio was significantly (p<0.01) increased in rice fed groups compared to corn fed groups. The supplementation of probiotics in both rice and corn based diets significantly (p<0.01) increased the villi height of duodenum, jejunum and ileum and VH: CD ratio in jejunum and ileum. It could be concluded that the dietary supplementation of probiotics at 0.1 per cent level was effective in reducing the intestinal E. coli, coliform count, E. faecalis count, ileal pH and improving the intestinal histo-morphometry. Dietary replacement of 50 per cent corn by rice had no significant effect on these parameters.
Keywords: Rice, probiotics, microbial count, morphometry
Alvarado, J.M.G., Moreno, E.J., Valencia, D.G., Lazaro, R. and Mateos, G.G. 2008. Effects of fiber source and heat processing of the cereal on the development and pH of the gastrointestinal tract of broilers fed diets based on corn or rice. Poult. Sci. 87: 1779-1795.
AOAC [Association of Official Analytical Chemists]. 2005. Official Method of Analysis. (18th Ed.). Association of Official Analytical Chemists, Washington, D. C., 771p.
BIS [Bureau of Indian Standards]. 2007. Poultry Feeds Specification. (5th Ed.). Bureau of Indian Standards, New Delhi, pp. 3-5.
Diaz Carrasco, J.M., Casanova, N.A. and Miyakawa, F.M.E. 2019. Microbiota, gut health and chicken productivity: What is the connection? Microorganisms. 7: 374.
Dhama, K., Verma, V., Sawant, P.M., Tiwari, R., Vaid, R.K. and Chauhan, R.S. 2011. Applications of probiotics in poultry: Enhancing immunity and beneficial effects on production performances and health- A review. J. Immunol. Immunpath. 13: 1-19.
Gridley, M.F. 1960. Manual of histologic and special staining techniques. McGraw-Hill INC, New York, 86p.
Gunal, M., Yayli, G., Kaya, O., Karahan, N. and Sulak, O. 2006. The effects of antibiotic growth promoter, probiotic or organic acid supplementation on performance, intestinal microflora and tissue of broilers. Int. J. Poult. Sci. 5: 149-155.
Izat, A.L., Tidwell, N.M., Thomas, R.A., Reiber, M.A., Adams, M.H., Colberg, M. and Waldroup, P.W. 1990. Effects of a buffered propionic acid in diets on the performance of broiler chickens and on microflora of the intestine and carcass. Poult. Sci. 69: 818-826.
Kim, J.S., Ingale, S.L., Kim, Y.W., Kim, K.H., Sen, S., Ryu, M.H., Lohakare, J.D., Kwon, I.K. and Chae, B.J. 2012. Effect of supplementation of multi‐microbe probiotic product on growth performance, apparent digestibility, caecal microbiota and small intestinal morphology of broilers. J. Anim. Physiol. Anim. Nutr. 96: 618-626.
Nabizadeh, A., Golian, A., Hassanabadi, A. and Zerehdaran, S. 2018. Effects of isolated soy protein and broken rice in corn-soy pre-starter diet on performance, intestinal microflora, and gut morphology in broiler chickens. J. Appl. Poult. Res. 27: 133–144.
Sen, S., Ingale, S.L., Kim, Y.W., Kim, J.S., Kim, K.H., Lohakare, J.D., Kim, E.K., Kim, H.S., Ryu, M.H., Kwona, I.K. and Chae, B.J. 2012. Effect of supplementation of Bacillus subtilis LS 1-2 to broiler diets on growth performance, nutrient retention, caecal microbiology and small intestinal morphology. Res. Vet. Sci. 93: 264–268.
Sittiya, J., Yamauchi, K. and Takata, K. 2015. Effect of replacing corn with whole-grain paddy rice and brown rice in broiler diets on growth performance and intestinal morphology. J. Anim. Physio. Anim. Nutr. 100: 381-390.
Supriya, R., Chacko, B., Anitha, P., Suja, C.S. and Priya, P.M. 2022. Effects of fermented soyabean meal, probiotics and organic acids on intestinal morphology, pH and microbial count in broilers. J.Vet.Anim.Sci. 53: 348-355.
© 2023 Reshma et al. This is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Reshma, R., Chacko, B., Anitha, P., Prasoon, S. and Chandrasekhar, L. 2023. Effect of probiotics and rice on ileal microbial count and intestinal morphometry in broiler chicken. J. Vet. Anim. Sci. 54(2):443-449
DOI: https://doi.org/10.51966/jvas.2023.54.2.443-449