Volume: 52 Issue: 4
Year: 2021, Page: 389-392, Doi: https://doi.org/10.51966/jvas.2021.52.4.389-392
Received: March 14, 2021 Accepted: May 19, 2021 Published: Dec. 15, 2021
India is endemic to bovine brucellosis, and there is a high potential for transmission of disease from ruminants to dogs. A total of 18 bitches belonging to five different breeds at different stage of abortion (30 days to 65 days of gestation) were selected for this study. Majority of them were showing abortion (88.89 per cent) at 45 to 65 days of the gestation. Microscopic examination of Stamp stained smear obtained from the aborted foetal stomach contents revealed red coccobacillary organisms suggestive of Brucella spp.in three cases. RBPT on paired sera samples on day of presentation and three weeks after abortion showed agglutination within four minutes in five out of 18 female dogs. DNA extracted from the aborted tissues of a RBPT positive Labrador dog yielded amplicons of 193 base pair specific for Brucella spp. on PCR. The results obtained from this study stress the need for screening dogs for canine brucellosis in the current brucellosis surveillance and control programmes.
Keywords: Bovine brucellosis, abortion, zoonotic disease, stamp staining, RBPT, PCR
Alamian, S. and Dadar, M. 2020. Brucella melitensis infection in dog: a critical issue in the control of brucellosis in ruminant farms. Comp. Immunol. Microbiol. Infect. Dis. 73: 1-5. Available: https://www. sciencedirect.com/science/article/pii/ S0147957120301430. [7 Mar. 2021].
Alton, G. G., Jones, L. M., Angus, R. D., Verger, J. M. 1988. Techniques for the brucellosis laboratory. (1st Ed.). Paris: Institut National de la Recherche Agronomique. 192p.
Baek, B. K., Lim, C.W., Rahman, M. S., Kim, C., Oluoch, A. and Kakoma, I. 2003. Brucella abortus infection in indigenous Korean dogs. Canad. J. Vet. Res. 67: 312- 314.
Behzadi, M. A. and Mogheiseh, A. 2011. Epidemiological survey of Brucella canis infection in different breeds of dogs in Fars province, Iran. Pak. Ve.t J. 32: 234- 236.
Bramlage, D. J., Fortney, W., Kesler, R. M. 2015. Best Practices for Brucella canis Prevention and Control in Dog Breeding Facilities. United States Department of Agriculture Animal and Plant Health Inspection Service. 2p.
Carmichael, L. E. 2018. Brucella canis. In: Nielsen, K. and Duncan, J. R. (ed.), Animal Brucellosis. (1st Ed.). CRC Press, Taylor & Francis group, London, pp. 335- 350.
Cosford, K. L. 2018. Brucella canis: An update on research and clinical management. Can. Vet. J. 59:74–81.
Hollett, R. B., 2006. Canine brucellosis: outbreaks and compliance. Theriogenology. 66: 575-587.
Leal-Klevezas, D. S., Martinez-Vaquez, I. O., Lopez-Merino, A. and Martinez-Soriano, J. P. 1995. Single-step PCR for detection of Brucella spp. from blood and milk of infected animals. J. Clin. Microbiol. 33: 3087–3090.
Mol, J. P., Guedes, A.C., Eckstein, C., Quintal, A. P., Souza, T. D. et al. 2020. Diagnosis of canine brucellosis: comparison of various serologic tests and PCR. J. Vet. Diagn. Invest. 32:77–86.
Pillai, M. T., Nedunchelliyan, S. and Raghavan, N. 1991. Serological and bacteriological detection of Brucella canis infection of dogs in Madras. Indian Vet. J. 68: 399- 401.
The centre for food security and public health. 2018. Canine brucellosis: Brucella canis. Iowa State University, Ames, Iowa.10p. Available: https://www.cfsph.iastate. edu/Factsheets/pdfs/brucellosis_canis. pdf
Wanke, M. M. 2004. Canine brucellosis. Anim. Reprod. Sci. 82-83: 195 -207.
© 2021 Athira et al. This is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Athira, K., Shyma, V. H., Justin, K. D., Vijayakumar, K. and Jayakumar, C. 2021. Epidemiological investigation for brucellosis in dogs of Thrissur. J. Vet. Anim. Sci. 52(4): 389-392
DOI: https://doi.org/10.51966/jvas.2021.52.4.389-392