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Journal of Veterinary and Animal Sciences

Volume: 48 Issue: 2

  • Open Access
  • Research Article

EVALUATION OF RAPID QUANTITATIVE LATEX ASSAY TO DETECT NEWCASTLE DISEASE VIRUS IN CHICKEN

K .P. Deepa1, P.M Priya 2 and M. Mini3

Department of Veterinary Microbiology, College of Veterinary and Animal Sciences, Mannuthy-680651, Thrissur, Kerala

Year: 2017, Page: 76-80,

Received: Oct. 22, 2016 Accepted: Nov. 23, 2016 Published: Dec. 31, 2017

Abstract

The present study was undertaken to evaluatethe efficacy of a rapid quantitative latex assay to detect Newcastle disease virus (NDV)
in birds. Out of 28 clinical samples collected from disease suspected birds, haemagglutination test detected 12 samples as positive and were confirmed as NDV by HI test. The latex agglutination test (LAT) was standardized and only 6 samples were found to be positive. The HA titre ranged from 1:8 to 1:128 and LAT titres were 1:2 in all LAT positive samples. Based on the statistical analysis, there exists a significant difference (p<0.05) between the two tests employed and HA-HI tests was found to be having higher sensitivity than LAT.

Keywords: Newcastle disease virus, haemagglutination, haemagglutination inhibition, latex agglutination test, Kerala.

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