Volume: 53 Issue: 2
Year: 2022, Page: 208-213, Doi: https://doi.org/10.51966/jvas.2022.53.2.208-213
Received: July 14, 2021 Accepted: Sept. 15, 2021 Published: June 30, 2022
There is a great demand of suitable biomaterials for tissue engineering applications in regenerative medicine. Decellularized extracellular matrix scaffolds derived from different organs and tissues have been successfully applied for human use as therapeutic agents in many tissue defects. The aim of the present study was to establish histological and ultrastructural characterisation as minimal criteria for assessing the suitability of decellularised bioscaffolds in tissue engineering applications. The study was performed on bovine omentum processed by a natural bovine bile based decellularisation protocol. Histological analysis and scanning electron microscopy examinations were performed and compared with an established decellularised bovine pericardial scaffold. Gross examination revealed that the decellularisation process did not alter the shape of the omentum. Histological examination confirmed the preservation of the collagenous fibres without any observable cell nuclei following decellularisation. Further, scanning electron microscopy examination demonstrated the arrangement and ultrastructure of collagen fibrils in the bioscaffolds. Our results suggest that histological and ultrastructural characterisation provide preliminary data on determining the biocompatibility of scaffolds before planning in vivo studies in tissue engineering applications.
Keywords: Biomaterial, bovine omentum, decellularisation
Aamodt, J.M. and Grainger, D.W. 2016. Extracellular matrix-based biomaterial scaffolds and the host response. Biomaterials. 86: 68-82.
Allen, R.A., Seltz, L.M., Jiang, H., Kasick, R.T., Sellaro, T.L. and Badylak, S.F. 2010. Adrenal extracellular matrix scaffolds support adrenocortical cell proliferation and function in vitro. Tissue. Eng. Part. A. 16 (11): 3363-3374.
Ashna, S., Dhanush Krishna, B., Sajitha, I.S., Vasudevan, V.N., Pavan, M. and Abraham, M.J. 2019. Effect of gamma ray sterilisation on differently de-cellularised omentum based scaffolds. Explor. Anim. Med. Res. 9 (2): 174-179.
Azum, N., Rub, M.A. and Asiri, A.M. 2019. Bile salt–bile salt interaction in mixed monolayer and mixed micelle formation. J. Chem. Thermodyn. 128: 406-414.
Balakrishnan Nair, D.K., Nair, N.D., Venugopal, S.K., Das, V.N., George, S., Abraham, M.J., Eassow, S., Alison, M.R., Sainulabdeen, A. and Anilkumar, T.V. 2018. An immunopathological evaluation of the porcine cholecyst matrix as a muscle repair graft in a male rat abdominal wall defect model. Toxicol. Pathol. 46: 169-183.
Balakrishnan Nair, D.K., Nair N.D., Venugopal, S.K., Abraham, M.J., Sunanda, C. and Anilkumar, T.V. 2019. Herovici’s staining: a useful differential staining method for evaluating collagen distribution in biomaterial-mediated hernia repair. J. Vet. Anim. Sci. 50: 154-158.
Chen, R.N., Ho, H.O., Tsai, Y.T. and Sheu, M.T. 2004. Process development of an acellular dermal matrix (ADM) for biomedical applications. Biomaterials. 25: 2679-2686.
Dussoyer, M., Michopoulou, A. and Rousselle, P. 2020. Decellularized scaffolds for skin repair and regeneration. J. Appl. Sci. 10: 3435p.
Fernandez-Perez, J. and Ahearne, M. 2019. The impact of Decellularisation methods on extracellular matrix derived hydrogels. Sci. Rep. 9(1): 1-12.
He, Y., Chen, Y., Wan, X., Zhao, C., Qiu, P., Lin, X., Zhang, J. and Huang, Y. 2020. Preparation and characterization of an optimized meniscal extracellular matrix scaffold for meniscus transplantation. Front. Bioeng. and Biotechnol. 8: 779p.
Lin, K., Zhang, D., Macedo, M.H., Cui, W., Sarmento, B. and Shen, G. 2019. Advanced collagen based biomaterials for regenerative biomedicine. Adv. Funct. Mater. 29(3): 1804943p.
Panwar, R., Pal, S., Dash, N.R., Sahni, P., Vij, A. and Misra, M.C. 2016. Why are we poor organ donors: a survey focusing on attitudes of the lay public from northern India. J. Clin. Exp. Hepatol. 6: 81-86.
Porzionato, A., Sfriso, M.M., Macchi, V., Rambaldo, A., Lago, G., Lancerotto, L., Vindigni, V. and Caro, R. 2013. Decellularized omentum as novel biologic scaffold for reconstructive surgery and regenerative medicine. Eur. J. Histochem. 57: 24–30.
Salbach, J., Kliemt, S., Rauner, M., Rachner, T.D., Goettsch, C., Kalkhof, S., von Bergen, M., Möller, S., Schnabelrauch, M., Hintze, V. and Scharnweber, D. 2012. The effect of the degree of sulfation of glycosaminoglycans on osteoclast function and signaling pathways. Biomaterials. 33: 8418-8429.
Sheridan, W.S., Duffy, G.P. and Murphy, B.P. 2012. Mechanical characterization of a customized decellularized scaffold for vascular tissue engineering. J. Mech. Behav. Biomed. Mater. 8: 58-70.
Suvarna, K., Layton, C. and Bancroft, J. 2018. Bancroft's Theory and Practice of Histological Techniques. (8th Ed.). Elsevier health sciences, 672p.
Vasudevan, V.N., Shakeer, M.T., Vidya, S.A., Rasmi, V.A., Neethu, R. and Sathu, T. 2016. Bovine gall bladder bile: a novel decellularizing agent for development of extracellular matrices. National Symposium Conference of Indian Meat Science Association: 10th to 12th November, 2016, Ludhiana. p192. Abstract No. IAPEP.
Wong, M.L. and Griffiths, L.G. 2014. Immunogenicity in xenogeneic scaffold generation: antigen removal vs. Decellularisation. Acta. Biomater. 10: 1806-1816.
Xing, Q., Vogt, C., Leong, K.W. and Zhao, F. 2014. Highly aligned nanofibrous scaffold derived from decellularized human fibroblasts. Adv. Funct. Mater. 24: 3027-3035.
Xing, Q., Yates, K., Tahtinen, M., Shearier, M., Qian, Z. and Zhao, F. 2015. Decellularisation of Fibroblast Cell Sheets for Natural Extracellular Matrix Scaffold Preparation. Tissue. Eng. Part. C. Methods. 21: 77–87.
© 2022 Megha et al. This is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Megha, K.G., Balakrishnan Nair, D.K., Divya, C., Sajitha, I.S., Vasudevan, V.N., Umashankar, P.R. and Devi, S.S. 2022. Histological and ultrastructural characterisation as minimal criteria for assessing the success of the decellularisation protocols for tissue engineering applications. J. Vet. Anim. Sci. 53(2): 208-213
DOI: https://doi.org/10.51966/jvas.2022.53.2.208-213