Volume: 53 Issue: 4
Year: 2022, Page: 651-656, Doi: https://doi.org/https://doi.org/10.51966/jvas.2022.53.4.651-656
Received: June 18, 2022 Accepted: Sept. 19, 2022 Published: Dec. 31, 2022
A study was conducted to identify virulence genes of Pseudomonas spp. from 20 cases of canine otitis externa in Wayanad district of Kerala. The samples were collected aseptically and bacterial isolates were identified using culture and biochemistry. The nine identified Pseudomonas isolates were selected and further confirmed by PCR, targeting Opr I gene at genus level. Presence of virulence genes viz. Las A, Las B, Lec A, Lec B and Apr were examined in the confirmed isolates. All the samples were positive for Las B and Apr. Five among the isolates were having Lec A gene. None of the isolates possessed Lec B and Las A gene. Presence of Las B and Apr gene indicate biofilm forming ability of the isolated Pseudomonas organisms.
Keywords: Canine otitis externa, Pseudomonas, PCR, virulence genes
Copyright: © 2022 Sacharia et al. This is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Sacharia,R.G., Bipin, K.C., Deepa, P.M., Rathish, R.L. and Ravishankar, C. 2022. Identification and detection of virulence genes among Pseudomonas spp isolated from Wayanad. J. Vet. Anim. Sci. 53(4): 651-656
DOI: https://doi.org/10.51966/jvas.2022.53.4.651-656