Journal of Veterinary and Animal Sciences

Volume: 54 Issue: 4

  • Open Access
  • Research Article

Influence of season in Kerala on the yield and quality of bovine oocytes obtained by ultrasound-guided transvaginal oocyte recovery#

E. Niyas1*, R. S Abhilash2, C. Jayakumar3, M. P. Unnikrishnan2, T. V. Aravindakshan4 and K. M. Shyam Mohan5

# Part of Ph.D. thesis submitted to Kerala Veterinary and Animal Sciences University, Pookode, Wayanad, Kerala


1 Ph.D. scholar
2 Assistant Professor
3 Associate professor and Head
4 Director, CASAGB
5 Professor and Head, ULF & FRDS, Mannuthy


*Corresponding author: [email protected], Ph. 9447265844

Year: 2023, Page: 934-943, Doi: https://doi.org/10.51966/jvas.2023.54.4.934-943

Received: March 12, 2023 Accepted: June 9, 2023 Published: Dec. 31, 2023

Abstract

The present study was carried out to assess the influence of three seasons in Kerala on the yield and quality of oocytes from crossbred (CB) dairy cows (n=27), subjected to ultrasound-guided transvaginal oocyte recovery (TVOR). Meteorological data including, ambient temperature (AbT) and relative humidity (RH) was recorded and Temperature Humidity Index (THI) was calculated. In each season, selected animals (n=9) were subjected to TVOR; data regarding the number of follicles available for puncture (>2mm), number of aspirated follicles, number of oocytes retrieved and oocyte recovery rate were recorded. The retrieved oocytes were subjected to morphological evaluation. Significantly higher (P<0.05) AbT and THI was recorded during summer (28.92±0.64oC and 80.61±0.76) when compared to rainy (26.95±0.17oC and 78.68±0.21) and post-monsoon (27.29±0.22 oC and 77.75 ± 0.57) seasons. However, due to the availability of rainfall throughout the year, RH did not differ significantly between seasons. The mean number of follicles imaged (available for puncture) by ultrasonography per session was significantly higher (P<0.01) during the rainy season (11.19±0.74) compared to post-monsoon (8.08±0.47) and summer (5.28±0.38). Total mean number of follicles aspirated and oocytes retrieved pe session was highest during rainy season (7.86±0.49 and 3.17±0.28) and least during summer (4.36±0.29 and 1.81±0.20). However, the oocyte recovery rate and the yield of culture-grade oocytes did not differ significantly between seasons. It was observed that the THI during all the seasons was above the thermo-comfortable level suggested for dairy cows. Ovarian activity of CB dairy cows was compromised during the summer season in Kerala, as evidenced by the lowest number of follicles aspirated and oocytes retrieved per session.

Keywords: Season, crossbred cows, ambient temperature, temperature humidity index, transvaginal oocyte recovery

References

Abdoon, A. S. S. and Kandil, O.M. 2001. Factors affecting number of surface ovarian follicles and oocytes yield and quality in Egyptian buffaloes. Repr. Nutr. Dev. 41: 71-77.

Abhilash, R. S. 2017. Yield and quality of transvaginally retrieved oocytes in normal and repeat breeding cattle. PhD thesis. Kerala Veterinary and Animal Sciences University, Pookode. 125p.

Abhilash, R.S., Joseph, M., Kurien, M.O., Harshan, H.M., Aravindakshan, T.V., Anil, K.S. and Gleeja, V.L. 2017. Impact of frequency of transvaginal oocyte recovery on ovarian biometry and oocyte yield in crossbred cattle of Kerala. Int. J. Sci. Env. Tech. 6: 2766 – 2770.

Alanteena, J. 2016. In vitro fertility assessment of frozen bovine spermatozoa as influenced by breed and season. M.V. Sc thesis, Kerala Veterinary and Animal Sciences University, Pookode. 95p.

Annual Report. 2011-12. Department of Animal Husbandry, Dairying and Fisheries. Ministry of Agriculture, Govt. of India, New Delhi.

Annual Report. 2019. Department of Animal Husbandry, Dairying and Fisheries. Ministry of Agriculture, Govt. of India, New Delhi.

Badinga, L., Thatcher, W.W., Diaz, T., Drost, M. and Wolfenson, D. 1993. Effect of environmental heat stress on follicular development and steroidogenesis in lactating Holstein cows. Theriogenology. 39: 797-810.

Bernabucci, U., Biffani, S., Buggiotti, L., Vitali, A., Lacetera, N. and Nardone, A. 2014. The effects of heat stress in Italian Holstein dairy cattle. J. Dairy Sci. 97: 471-486.

Biya, A.J. 2011. Assessment and alleviation of environmental stress on productive performance of rabbits. PhD thesis, Kerala Veterinary and Animal Sciences University, Pookode, 163p.

Camargo, L.S.A., Viana, J.H.M., Ramos, A.D.A., Serapiao, R.V., De Sa, W.F., Ferreira, A.D.M., Guimaraes, M.F.M. and do Vale Filho, V.R. 2007. Developmental competence and expression of the Hsp 70.1 gene in oocytes obtained from Bos indicus and Bos taurus dairy cows in a tropical environment. Theriogenology. 68: 626-632.

Campbell, B.K. 2018. The endocrine and local control of ovarian follicle development in the ewe. Anim. Reprod. 6: 159-171.

Chrenek, P., Kubovicova, E., Olexikova, L., Makarevich, A.V., Toporcerova, S. and Ostro, A. 2015. Effect of body condition and season on yield and quality of in vitro produced bovine embryos.

Zygote. 23:893-899. Garcia, A. and Salaheddine, M. 1998. Effects of repeated ultrasound-guided transvaginal follicular aspiration on bovine oocyte recovery and subsequent follicular development. Theriogenology. 50. 575- 585.

Gendelman, M. and Roth, Z. 2012. In vivo vs. in vitro models for studying the effects of elevated temperature on the GV-stage oocyte, subsequent developmental competence and gene expression. Anim. Reprod. Sci. 134: 125-134.

Goodhand, K.L., Watt, R.G., Staines, M.E., Hutchinson, J.S.M. and Broadbent, P.J. 1999. In vivo oocyte recovery and in vitro embryo production from bovine donors aspirated at different frequencies or following FSH treatment. Theriogenology. 51: 951-961.

ICAR [ Indian Council of Agricultural Research]. 1977. Handbook of Animal Husbandry. Third edition. Indian Council of Agricultural Research, New Delhi, 381p.

Kim, D., Kim, N., Kim, S., Kim, C.L., Jeon, I.S., Kim, M. and Ko, Y. 2018, November. Seasonal effect of Hanwoo oocyte recovery & embryo development by OPU technology. In The 18th International Symposium on Developmental Biotechnology. pp. 172-172.

LPHSI [Livestock and Poultry Heat Stress Indices]. 1990. The livestock and poultry heat stress indices for cattle, sheep and goats. In: the agriculture engineering technology guide, Clemson University, Clemson, SC, USA.

Manik, R.S., Singla, S.K. and Palta, P. 2003. Collection of oocytes through transvaginal ultrasound-guided aspiration of follicles in an Indian breed of cattle. Anim. Rep. Sci. 76: 155–161.

NICRA [National Initiative on Climate Resilient Agriculture]. 2012. National initiative on climate Resilient Agriculture. Research highlights, 2012-13.Central Research Institute of Dryland Agriculture. Hyderabad.

Nishant, P. 2009. Management protocol for averting drop of milk production in dairy cattle during summer. M.V.Sc. thesis, Kerala Agricultural University, Thrissur, 65p.

Prasad, A. 2014. Climatic adaptation and stress evaluation of crossbred cattle of Kerala. Ph D thesis. Kerala Veterinary and Animal Sciences University. 163p.

Revathy, M.M., Abhilash, R.S., Jayakumar, C., Magnus, P.K., Raji, K., James, J. and Kurien, M.O. 2021. In vitro fertilisation capacity of frozen crossbred bull semen cryopreserved during different seasons in Kerala. Indian J. Anim. Res. 56:1488- 1491.

Roth, Z. 2017. Effect of heat stress on reproduction in dairy cows: insights into the cellular and molecular responses of the oocyte. A. Rev. Anim. Biosci.5:151- 170.

Roth, Z., Arav, A., Bor, A., Zeron, Y., Braw-Tal, R. and Wolfenson, D. 2001. Improvement of quality of oocytes collected in the autumn by enhanced removal of impaired follicles from previously heat-stressed cows. Reprod. Camb. 122: 737-744.

Roth, Z., Meidan, R., Braw-Tal, R. and Wolfenson, D. 2000. Immediate and delayed effects of heat stress on follicular development and its association with plasma FSH and inhibin concentration in cows. J. Reprod. Fert. 120: 83-90.

Silva, C.F., Sartorelli, E.S., Castilho, A.C.S., Satrapa, R.A., Puelker, R.Z., Razza, E.M., Ticianelli, J.S., Eduardo, H.P., Loureiro, B. and Barros, C.M. 2013. Effects of heat stress on development, quality and survival of Bos indicus and Bos taurus embryos produced in vitro. Theriogenology. 79: 351-357.

Snedecor, G.W. and Cochran, W.G. 1994. Statistical methods. Affiliated East. West Press, New Delhi. 13:1467-1473.

Somanathan, V.L. 1980. Bioclimatological studies on dry matter intake and water consumption of growing livestock. M.V.Sc. thesis, Kerala Agricultural University.

Wolfenson, D. and Roth, Z. 2019. Impact of heat stress on cow reproduction and fertility. Anim. Frontiers. 9:32-38.

Zeron, Y., Ocheretny, A., Kedar, O., Borochov, A., Sklan, D. and Arav, A. 2001. Seasonal changes in bovine fertility: relation to developmental competence of oocytes, membrane properties and fatty acid composition of follicles. Reproduction. 121: 447–454

Cite this article

Niyas, E., Abhilash, R.S., Jayakumar, C., Unnikrishnan, M.P., Aravindakshan, T.V. and Shyam Mohan, K.M. 2023. Influence of season in Kerala on the yield and quality of bovine oocytes obtained by ultrasound-guided transvaginal oocyte recovery. J. Vet. Anim. Sci. 54(4):934-943

Views
424
Downloads
185
Citations