Volume: 54 Issue: 2
Year: 2023, Page: 600-603, Doi: https://doi.org/doi.org/10.51966/jvas.2023.54.2.600-603
Received: Nov. 9, 2022 Accepted: Feb. 20, 2023 Published: June 30, 2023
Corneal ulcers are one of the most common eye affection in domestic animals, especially dogs. In preclinical studies, porcine corneas were used regularly as an animal model due to its relative similarity to human cornea and its availability in great numbers from slaughter houses. In the present study, decellularised porcine cornea was used for corneal grafting as an acellular scaffold graft material in a case presented with deep corneal ulcer. The efficiency of the graft material was analysed on the day of presentation, days 7, 14, 21 and 60 post grafting, based on corneal clarity, oedema, neovascularisation, pigmentation, visual function and scar tissue formation. Decellularised porcine corneas were found to be very convenient to handle during surgery, provided good tectonic support, had good tissue biocompatibility and produced only mild to moderate graft opacity.
Keywords: Decellularised porcine cornea, deep corneal ulcers, grafting
Adarsh, A.M., John Martin, K.D., Devanand, C.B., Anoop, S., Unnikrishnan, M.P., Laiju Philip, M. and Soumya, R. 2016. Effect of N-Butyl-Cyanoacrylate tissue adhesive on canine corneal ulcers. J. Vet. Anim. Sci. 48: 43-46.
Amon, M. and Busin, M. 2012. Loteprednol etabonate ophthalmic suspension 0.5%: efficacy and safety for postoperative anti-inflammatory use. Int. Ophthalmol. 32: 507-517.
Anoop, S., Pallavi, K.S., Eassow, S., Venugopal, S.K., Joyous, T.V., Devanand, C.B. and Anilkumar, T.V. 2017. Cholecyst derived collagen as an extracellular matrix scaffold graft for the management of corneal injuries in dogs: a report of three cases. Indian J. Vet. Surg. 38: 55-56.
Antonia, N.A., Narayanan, M.K., Anoop, S., Devanand, C.B., Martin, J. and Venugopal, S.K. 2014. Occurrence of ophthalmic disorders in dogs. Indian. J. Vet. Res. 23: 21-24.
Azoulay, T. 2014. Adjunctive cryotherapy for pigmentary keratitis in dogs: a study of 16 corneas. Vet. Ophthalmol. 17: 241-249.
Bouhanna, L., Liscoët, L.B. and Raymond‐Letron, I. 2008. Corneal stromal sequestration in a dog. Vet. Ophthalmol. 11: 211-214.
Chow, D.W. and Westermeyer, H.D. 2016. Retrospective evaluation of corneal reconstruction using ACell Vet™ alone in dogs and cats: 82 cases. Vet. Ophthalmol.19: 357-366.
Goulle, F. 2012. Use of porcine small intestinal submucosa for corneal reconstruction in dogs and cats: 106 cases. J. Small Anim. Pract. 53: 34-43.
Isidan, A., Liu, S., Chen, A.M., Zhang, W., Li, P., Smith, L.J., Hara, H., Cooper, D.K. and Ekser, B. 2021.Comparison of porcine corneal decellularization methods and importance of preserving corneal limbus through decellularization. Plos one. 16:e0243682.
Jhanji, V., Young, A.L., Mehta, J.S., Sharma, N., Agarwal, T. and Vajpayee, R.B. 2011. Management of corneal perforation. Surv. Ophthalmol. 56: 522-538.
Keenan, A.V., Boveland, S.D., Rodriguez Galarza, R. and Moore, P.A. 2020.Corneoconjunctival transposition with and without ACell® for deep corneal ulcer repair in 18 dogs. Vet. Ophthalmol.23: 884-891.
Labelle, A.L., Dresser, C.B., Hamor, R.E., Allender, M.C. and Disney, J.L. 2013. Characteristics of, prevalence of, and risk factors for corneal pigmentation (pigmentary keratopathy) in Pugs. J. Am. Vet. Med. Assoc. 243: 667-674.
Lavaud, A., Kowalska, M.E., Voelter, K., Pot, S.A. and Rampazzo, A. 2021. Penetrating Keratoplasty in Dogs using Acellular Porcine Corneal Stroma (BioCorneaVet™): A prospective pilot study of five cases. Vet. Ophthalmol. 24: 543-553.
Moore, P.A. 2003. Diagnosis and management of chronic corneal epithelial defects (indolent corneal ulcerations). Clin. Tech. Small Anim. Pract. 18: 168-177.
Renwick, P. 1996. Diagnosis and treatment of corneal disorders in dogs. In Pract. 18: 315-328.
Startup, F.G. 1984. Corneal ulceration in the dog. J. Small Anim. Pract. 25: 737-752.
Suhas, K.P. 2015. Evaluation of porcine cholecyst derived collagen scaffold for the treatment of corneal injuries in dogs. M.V.Sc. Thesis. Kerala Veterinary and Animal Sciences University, Pookode. 116p.
Thajunnisa, A.S., Sainulabdeen, A., Dileepkumar, K.M., Philip, L.M., Vasudevan, V.N. and Devanand, C.B. 2020. Comparative evaluation of decellularized bovine omentum alone and in combination with mitomycin-C in the management of corneal injuries in dogs. Vet. World. 13: 2401-2410.
Vanore, M., Chahory, S., Payen, G. and Clerc, B. 2007. Surgical repair of deep melting ulcers with porcine small intestinal submucosa (SIS) graft in dogs and cats. Vet. Ophthalmol. 10: 93-99.
Wilkie, D.A. and Whittaker, C. 1997. Surgery of the cornea. Vet. Clin. : Small Anim. Pract. 27: 1067-1107.
© 2023 Aswathy et al. This is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Aswathy, M.A., Anoop, S., John, M.K.D., Laiju M.P., Vasudevan, V.N. and Ashlin, M. 2023. Lamellar keratoplasty using decellularised porcine cornea as an acellular scaffold graft for surgical management of deep corneal ulcer in a dog. J. Vet. Anim. Sci. 54(2):600-603
DOI: https://doi.org/10.51966/jvas.2023.54.2.600-603