Volume: 49 Issue: 1
Year: 2018, Page: 6-8,
Received: Feb. 15, 2017 Accepted: June 7, 2017 Published: Jan. 1, 2018
Three ailing broilers of age 3 weeks, 5 weeks and 9 weeks respectively were presented to the Department of Veterinary Microbiology, College of Veterinary and Animal Sciences, Mannuthy from three different private farms in Thrissur district for disease investigation. Owners reported a high mortality rate among the flocks and the birds were showing symptoms such as anorexia, depression, ruffled feathers, white watery diarrhoea and death. On post mortem examination, enlargement of bursa suggestive of infectious bursal disease (IBD) was noticed. The further confirmation of the cases were done with a reverse transcriptase polymerase chain reaction (RT-PCR) targeting the VP2 gene of Infectious bursal disease virus (IBDV), which revealed an amplicon size of 480 bp, specific for VP2 gene.
Keywords: Infectious bursal disease; RT-PCR; Thrissur district
Cosgrove, A.S. 1962. An apparently new disease of chickens. Avian nephrosis. Avian Dis. 6:385-389.
Office International des Epizooties (OIE)Manual of Diagnostic Tests and Vaccines for Terrestrial Animals. 7th ed. Paris: Office International des Epizooties; 2012. Fowl typhoid and pullorum disease; pp. 1–14.
Okoye, J.O.A. and Uzoukwu, M. 1981. An outbreak of infectious bursal disease among chickens between 16 and 20 weeks old. Avian Dis. 25(4):1034–1038.
Singh, Y., Yadav, K., Singh, V.K. and Kumar, R. 2014. Molecular diagnosis and adaptation of highly virulent infectious bursal disease virus on chicken embryo fibroblast cell. Vet. World. 7(5):351-355.
Teshome, M. and Admassu, T. F. B. 2015. Infectious Bursal Disease (GUMBORO Disease) in Chickens. Br. Poult. Sci. 4 (1): 22-28.
Yousif, M. G. O. 2005. Evaluation of various techniques used for diagnosis of infectious bursal disease and its prevalence in Khartoum state. M.V.Sc thesis, University of Khartoum, Khartoum, 99p
© 2018 Thanksy S. Akkara et al This is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.