Volume: 48 Issue: 1
Year: 2017, Page: 6-10,
Received: June 26, 2016 Accepted: June 30, 2016 Published: June 30, 2017
Successful cryopreservation of oocyte using vitrification ensures availability of large number of oocytes for in vitro maturation (IVM) and in vitro fertilization (IVF).Ovaries were collected from 24 slaughtered cattle and culture grade oocytes were selected by aspiration. Group І consisted of thirty eight oocytes vitrified using cryoloop and matured in vitro, while group ІІ consisted of thirty three oocytes vitrified after maturation. The oocytes were evaluated for morphological damages and viability. In group І, 52.63 per cent oocytes were morphologically viable, whereas in group ІІ per cent of viable oocytes was 66.67. Viability per cent of live oocytes did not differ (P > 0.05) significantly between two groups. The results indicated that meiotic stage of oocyte had no influence on morphological survivability of bovine oocytes subjected to cryoloop vitrification
Keywords: Aspiration, cryoloop, bovine oocytes, viability
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