Volume: 54 Issue: 2
Year: 2023, Page: 486-491, Doi: https://doi.org/10.51966/jvas.2023.54.2.486-491
Received: Nov. 14, 2022 Accepted: Jan. 6, 2023 Published: June 30, 2023
Canine distemper, a highly fatal systemic disease in domestic dogs and wild carnivores, has the second highest mortality rate after rabies and is responsible for a large number of animal deaths around the world. It is considered a major pathogen in the canine infectious respiratory disease complex. This paper reports the finding of a study conducted to detect and characterise canine distemper virus (Canine Morbillivirus) based on N gene. A total of 59 samples collected from cases of respiratory infections in dogs were subjected to N gene based reverse transcriptase polymerase chain reaction (RT-PCR) and eleven of them (18.64 per cent) were found positive. Sequencing and phylogenetic analysis revealed that all the canine distemper viruses obtained in the present study were related to Indian strains that were previously reported. However, the viruses from the same district were similar among themselves.
Keywords: Canine distemper virus, phylogenetic analysis, reverse transcriptase-polymerase chain reaction, N gene
McCarthy, A.J., Shaw, M.A. and Goodman, S.J. 2007. Pathogen evolution and disease emergence in carnivores. Proc. R. Soc. B: Biol. Sci. 274: 3165-3174.
Loots, A.K., Mitchell, E., Dalton, D.L., Kotzé, A. and Venter, E.H. 2017. Advances in canine distemper virus pathogenesis research: a wildlife perspective. J. Gen. Virol. 98: 311-321.
Day, M.J., Carey, S., Clercx, C., Kohn, B., MarsilIo, F., Thiry, E., Freyburger, L., Schulz, B. and Walker, D.J. 2020. Aetiology of canine infectious respiratory disease complex and prevalence of its pathogens in Europe. J. Comp. Path. 176: 86-108.
Sidhu, M.S., Husar, W., Cook, S.D., Dowling, P.C. and Udem, S.A. 1993. Canine distemper terminal and intergenic non-protein coding nucleotide sequences: completion of the entire CDV genome sequence. Virol. 193: 66-72.
Beineke, A., Baumgärtner, W. and Wohlsein, P. 2015. Cross-species transmission of canine distemper virus—an update. One Hlth. 1:49-59.
Deem, S.L., Spelman, L.H., Yates, R.A. and Montali, R.J. 2000. Canine distemper in terrestrial carnivores: a review. J. Zoo Wildlife Med. 31: 441-451.
Frisk, A.L., Konig, M., Moritz, A. and Baumgartner, W. 1999. Detection of canine distemper virus nucleoprotein RNA by reverse transcription-PCR using serum, whole blood, and cerebrospinal fluid from dogs with distemper. J. Clin. Microbiol. 37: 3634-3643.
Yang, Z. 1997. PAML: a program package for phylogenetic analysis by maximum likelihood. Comp. Appl. Biosci. 13: 555-556.
Felsenstein, J.1985. Confidence limits on phylogeneis: An approach using the bootstrap. Evolution. 39: 783-791.
Tamura, K. 1992. Estimation of the number of nucleotide substitutions whenthere
are strong transition-transversion and G+C content biases. Mol. Biol.
Evol. 9: 678–687.
Bhatt, M., Rajak, K.K., Chakravarti, S., Yadav, A.K., Kumar, A., Gupta, V., Chander, V., Mathesh, K., Chandramohan, S., Sharma, A.K. and Mahendran, K. 2019. Phylogenetic analysis of haemagglutinin gene deciphering a new genetically distinct lineage of canine distemper virus circulating among domestic dogs in India. Transbound. Emerg. Dis. 66:1252-1267.
Deka, D., Uppal, S.K. and Verma, R. 2015. Isolation and phylogenetic characterization of Canine distemper virus from India. Virus Dis. 26: 133-140.
Ashmi, J.M., Thangavelu, A., Senthilkumar, T.M.A. and Manimaran, K. 2017. Molecular characterization of Canine distemper virus from Tamil Nadu, India. Indian J. Anim. Sci. 87: 1062–1067.
Pawar, R. M., Raj, G. D., Gopinath, V. P., Ashok, A. and Raja, A. 2011. Isolation and molecular characterization of Canine distemper virus from India. Trop. Anim. Hlth Prod. 43: 1617–1622.
Shin, Y.J., Cho, K.O., Cho, H.S., Kang, S.K., Kim, H.J., Kim, Y.H., Park, H.S. and Park, N.Y. 2004. Comparison of one‐step RT‐PCR and a nested PCR for the detection of canine distemper virus in clinical samples. Aust. Vet. J. 82: 83-86.
© 2023 Maneesh et al. This is an open access article distributed under the terms of the Creative
Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits
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Maneesh, K.M., Sumod, K., Ravishankar, C., John, K., Rajasekhar, R., Arun, P.M.,Shashank, S., Anjitha, G.S., Haridas, J.P., Jayanth, K.V., Raj. M.N. and Ramya, S.L. 2023. N gene based detection and phylogenetic analysis of canine morbillivirus in dogs. J. Vet. Anim. Sci.
54(2):486-491
DOI: https://doi.org/10.51966/jvas.2023.54.2.486-491