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Journal of Veterinary and Animal Sciences

Volume: 49 Issue: 2

  • Open Access
  • Short Communication

NOVEL TECHNIQUE OF SHEET PLASTINATION

Annie V. Raj1*, K. V. Jamuna2, Shruti3, Sunilkumar Patil4, V. Ramkrishna5 and C. Thandavamurthy6

1 PhD. Scholar, Department of Veterinary Anatomy and Histology, College of Veterinary and Animal Sciences, Mannuthy* Corresponding author email- [email protected], Ph: 9496366093 2 Prof. & Head 3 MVSc. Scholar 4 Phd. Scholar 5 Retd. Prof.& Head & cum contract teacher 6 Retd. Prof.& Head & cum contract teacher

Year: 2018, Page: 97-99,

Received: Nov. 30, 2017 Accepted: Jan. 6, 2018 Published: July 1, 2018

Abstract

The frozen brain was sliced at a thickness of 1 cm. Then the sections were placed in 10% formol saline and then dehydrated in 3 changes of acetone for 3 days. Fig. 3. Photograph showing delineation of grey and white mater of brain using berlin blue staining (A) and unstained (B Fig. 1. Photograph showing slicing of brain Fig. 2. Photograph showing the serially arranged section After dehydration sections were infiltrated with melted jelly wax for 5 minutes. All the infiltrated sections were placed in melted jelly wax and allowed to solidify. Then the sections were covered with glass sheets and serially arranged in a long glass sheet. Such specimen will be of a great use for the developing countries for the preparation of sheet plastination. This can be used for the detailed study of CT scan and MRI in the diagnostic method under veterinary medicine.

Keywords:

References

Barnett, R., Burland, G. and Duxson, M. 2005. Plastination of coronal slices of brainsfrom cadavers using the P 35 technique. J. Int. Soc. Plastination.20: 16-19.

Tompsett, D.H. 1970. Anatomical Techniques. 2nded., Edinburd and London publishers. pp. 242-247.

Von Hagens, G., Tiedemann, K. and Kriz, W. 1987. The current potential of plastination. Anat. Embryol.175: 411- 421.

Von Hagens, G. 1994. Plastination of brain slice according to the P40 procedure.A step by step description.pp. 1-23.

Weber, W. 1992. Sheet plastination of the brain, P35 technique, filling technique. J. Int. Soc.Plastination.6(1): 6-7.

Weber, W., Weiglein, A., Latorre, R. and Henry, R. W. 2007. PolysterPlastinationof biological tissue: P 35 Technique.J. Int. Soc.Plastination.22: 50-58.

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