Volume: 53 Issue: 1
Year: 2022, Page: 55-59, Doi: https://doi.org/10.51966/jvas.2022.53.1.55-59
Received: April 22, 2021 Accepted: June 8, 2021 Published: March 31, 2022
The present study was undertaken for the isolation and identification of Klebsiella pneumoniae and Salmonella spp. from environmental samples. A total of 120 soil and 120 water samples were collected from ponds and rivers in Thrissur. The samples were subjected to conventional culture techniques and K. pneumoniae and Salmonella spp. isolates were detected in 57.50 and 14.58 per cent samples, respectively.There were no significant differences between the occurrence of K. pneumoniae and Salmonella spp. in different soil and water samples, (p≥0.05). The isolates were subjected to molecular confirmation targeting rpoB gene for K. pneumoniae and invA gene for Salmonella spp. Out of the 138 K. pneumoniae and 35 Salmonella spp. isolates analysed, 86.96 and 97.14 per cent isolates were found positive on PCR. The results of the study revealed that environment acts as an important reservoir of K. pneumoniae and Salmonella spp.
Keywords: Klebsiella pneumoniae, Salmonella spp., environment, water
Amini, K., Salehi, T.Z., Nikbakht, G., Ranjbar, R., Amini, J. and Ashrafganjooei, S.B. 2010. Molecular detection of invA and spv virulence genes in Salmonella enteritidis isolated from human and animals in Iran. Afr. J. Microbiol. Res. 4: 2202-2210.
APHA [American Public Health Association]. 2012. Standard methods for the examination of water and waste water. (22nd Ed.). American Public Health Association, Washington. 1325p.
Bobbadi, S., Kiranmayi Chinnam, B., Nelapati, S., Tumati, S.R., Kandhan, S., Gottapu, C. and Boddu, S.V. 2020. Occurrence and genetic diversity of ESBL producing Klebsiella species isolated from livestock and livestock products. J. Food Safety. 40: 127-138.
Chaudhary, J.H., Nayak, J.B., Brahmbhatt, M.N. and Makwana, P.P. 2015. Virulence genes detection of Salmonella serovars isolated from pork and slaughterhouse environment in Ahmedabad, Gujarat. Vet. Wld. 8: 121-132.He, Y., Guo, X., Xiang, S., Li, J., Li, X., Xiang, H., He, J., Chen, D. and Chen, J. 2016. Comparative analyses of phenotypic methods and 16S rRNA, khe, rpoB genes sequencing for identification of clinical isolates of Klebsiella pneumoniae. Antonie Van Leeuwenhoek. 109: 1029-1040.
Grimont, P.A. and Weill, F.X., 2007. Antigenic formulae of the Salmonella serovars. WHO collaborating centre for reference and research on Salmonella, 9, pp.1-166.
He, Y., Guo, X., Xiang, S., Li, J., Li, X., Xiang, H., He, J., Chen, D. and Chen, J. 2016. Comparative analyses of phenotypic methods and 16S rRNA, khe, rpoB genes sequencing for identification of clinical isolates of Klebsiella pneumoniae. Antonie Van Leeuwenhoek. 109: 1029-1040.
Keelara, S., Scott, H.M., Morrow, W.M., Gebreyes, W.A., Correa, M., Nayak, R., Stefanova, R. and Thakur, S. 2013. Longitudinal study of distributions of similar antimicrobial-resistant Salmonella serovars in pigs and their environment in two distinct swine production systems. Appl. Environ. Microbiol. 79: 5167-5178.
Ko, W.C., Paterson, D.L., Sagnimeni, A.J., Hansen, D.S., Von Gottberg, A., Mohapatra, S., Casellas, J.M., Goossens, H., Mulazimoglu, L., Trenholme, G. and Klugman, K.P. 2002. Community-acquired Klebsiella pneumoniae bacteremia: global differences in clinical patterns. Emerg. Infect. Dis. 8: 160-169.
Kumar, D., Shrutikirti, A. and Kumari, K. 2013. Klebsiella: In drinking water. Int. J. Pharm. Sci. Invent. 12: 38-42.
Podschun, R., Pietsch, S., Höller, C. and Ullmann, U. 2001. Incidence of Klebsiella species in surface waters and their expression of virulence factors. Appl. Environ. Microbiol. 67: 3325-3327.
Tafoukt, R., Touati, A., Leangapichart, T., Bakour, S. and Rolain, J.M. 2017. Characterization of OXA-48-like-producing Enterobacteriaceae isolated from river water in Algeria. Wat. Res. 120: 185-189.
© 2022 Swetha et al. This is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Swetha, P.T., Binsy, M., Latha, C., and Sethulekshmi, C. 2022. Occurrence of Klebsiella pneumoniae and Salmonella spp. in environmental samples. J. Vet. Anim. Sci. 53(1): 55-59.
DOI: https://doi.org/10.51966/jvas.2022.53.1.55-59