Volume: 49 Issue: 2
Year: 2018, Page: 44-49,
Received: Aug. 8, 2017 Accepted: Aug. 14, 2017 Published: July 1, 2018
A study was undertaken to isolate the Salmonella serovars from gastroenteritic lesions of piglets and to assess the pathogenic effect of porcine Salmonella in gastrointestinal tract of mice. Tissue samples were collected from piglets showing varying degrees of gastroenteritis. The tissue samples were subjected to different conventional culture methods and biochemical tests for Salmonella serovar identification. Ten sample isolates were isolated from 90 samples and were identified as Salmonella Choleraesuis. The major virulent Salmonella isolate with infective dose of 2.7 x 107 CFU/ml was administered orally for pathogenesis study to 48 mice based on the pilot study along with a control group of six mice. Six mice each were sacrificed by cervical dislocation at 6, 12, 24, 36, 48, 60, 72 and 96 h, post-inoculation. Detailed postmortem examination was conducted and gross lesions particularly of gastrointestinal tract (GIT) were recorded. Gross lesions of mice revealed severe catarrhal enteritis, hepatic degeneration and pulmonary emphysema at varying intervals. The representative tissue samples of gastrointestinal tract were collected in 10 per cent neutral buffered formalin and processed for histopathological examination
Keywords: Piglets, Salmonella, Pathogenesis study, Mice
Bancroft, J.D., Gamble, M. 2007. Theory and Practice of Histological Techniques. 6th edn., Churchill Livingstone, United States of America. 725.
Ecco, R., Guedes, R. M. C., Tury, E., Junior, H. L.S. and Perecmanis, S. 2006. Outbreak of enterocolitic salmonellosis on a wild pig farm. Vet. Rec.158: 242-243.
Irimie, S.M., Herman, V., Pascu, C., Costinar, L., Faur, B., Surpat, A.S., Serbescu, M.,vaduva, I. 2010. Characterization of salmonella strains isolated from weaned pigs. Lucrări Stiinłifice Medicină Veterinară XL III (1), Timisoara.
Malik, M.A., Kotwal, S.K., Rahman, S.Q., Singh, M. and Sharma, N. 2015. Pathomorphological Changes in Various Organs of Experimentally Induced Salmonellosis in Mice. J. Anim. Res. 5: 605-609.
Molla, B., Berhanu, A., Muckle, A., Cole, L., Wilkie, E., Kleer, J., Hildebrandt, G. 2006. Multidrug Resistance and Distribution of Salmonella Serovars in Slaughtered Pigs. J. Vet. Med. 53: 28-33.
Perez, J., Astorga, R., Carrasco, L., Mendez, A., Perea, A. and Sierra, M.A. 1999. Outbreak of salmonellosis in farmed European wild boars (Sus scrofa ferus). Vet. Rec. 145: 464-465.
Reed, W.M., Olander, H.J. and Thacker, H.L. 1986. Studies on the pathogenesis of Salmonella Typhimurium and Salmonella Choleraesuis var kunzendorf infection in weanling pigs. Am J Vet Res. 47: 75-83.
Sakano, C., Morita1, Y., Goto, K., Yokota, Y., Annaka, H., Fujita, M., Kobatake, S., Ishioka, T., Hoshino, T., Boonmar, S., Pulsrikarn, C., Nishina, A., Kozawa,K.,Yamamoto, S. and Kimura1, H. 2011. Prevalence and Genotype of Salmonella Choleraesuis in Gunma Prefecture, Japan. Thai. J. Vet. Med. 41: 321-326.
Sundari, A.R., Chetty, M.S., Sreenivasulu, D., Srilatha, C. and Babu, A.J. 2011. Experimental toxicity studies of Salmonella serovars isolated from pigs, in mice and germinating seeds. Tamilnadu J. Vet. & Anim. Sci. 7: 97-101
© 2018 Prasad et al. This is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.