Volume: 53 Issue: 1
Year: 2022, Page: 112-116, Doi: https://doi.org/10.51966/jvas.2022.53.1.112-116
Received: April 4, 2021 Accepted: June 6, 2021 Published: March 31, 2022
Old world monkeys comprise 28 Langur species which belong to subfamily Colobinae under family Cercopithecidae. Nilgiri Langurs (Trachypithecus johnii) are endemic to the rain forests of the Western Ghats. The current study is an attempt at comparative phylogeny based on mitochondrial CYTB (mtCYTB) gene of Nilgiri Langur with other langurs. Faecal sample was collected from Nilgiri langur and the genomic DNA was isolated. The 1140 bp mitochondrial CYTB was amplified and sequenced using Sanger’s di-deoxy method. The amplified sequence along with the 27 sequences of Trachypithecus and Semnopithecus that were retrieved from the GenBank database were used for analysis. The phylogenetic tree was constructed by the maximum likelihood method in MEGA X. The analysis showed the clustering of Nilgiri langur with other langurs of the Semnopithecus sp. as a single clade.
Keywords: Mitochondria, Langur, CYTB, Trachypithecus, Semnopithecus, phylogeny
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© 2022 Meganath et al. This is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Meganath, K. V., Muhasin, A., Radhika, G., George, C., Manoj, M., Dinesh, C. N., Jacob, A., Muhammed, E. M. and Rojan, P. M. 2022. Phylogenetic analysis of Nilgiri langur using mitochondrial cytochrome B gene. J. Vet. Anim. Sci. 53(1): 112-116.
DOI: https://doi.org/10.51966/jvas.2022.53.1.112-116