Volume: 50 Issue: 1
Year: 2019, Page: 23-25,
Received: Aug. 10, 2017 Accepted: Oct. 13, 2017 Published: Jan. 1, 2019
The article describes the culture of microfilaria of Brugia spp. for the extraction of excretory-secretory protein. The microfilariae were separated from the various components of canine blood using HiSep™ LSM by gradient centrifugation method. The microfilariae were cultured in RPMI-1640 media and incubated at 37°C. The spent media was collected at 12 hour intervals, filtered, pooled and stored at -50°C. Protein estimation was done by Bradford’s method. The excretory-secretory protein produced can further be used in diagnostic tests.
Keywords: Excretory secretory protein, Brugiasp. HiSep, Canine
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© 2019 Poojary Vineeta Sadarama et al. This is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited