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Journal of Veterinary and Animal Sciences

Volume: 49 Issue: 1

  • Open Access
  • Research Article

PURIFICATION AND QUANTIFICATION OF FIBRONECTIN TYPE II PROTEINS IN MALABARI BUCK SEMINAL PLASMA

Keerthy G. Krishnan1, Hiron M. Harshan2, M. P.Unnikrishnan3, M.O.Kurien 4 and V. Ramnath 5

1. M.V.Sc. Scholar Email: [email protected], Phone no: 9400720992 2. Assistant Professor 3. Assistant Professor 4. Professor and Head 5. Controller of Examinations, KVASU.

Year: 2018, Page: 89-92,

Received: June 14, 2017 Accepted: June 17, 2017 Published: Jan. 1, 2018

Abstract

The study was conducted to purify and quantify Fn2 type (Fibronectin type II) proteins of Malabari buck seminal plasma. Quantification of whole seminal plasma protein did not reveal any significant difference between bucks with good and poor semen freezability, while significant difference was observed in the quantity of Fn2 proteins in the two groups of bucks. SDS-PAGE analysis revealed four gelatin binding proteins with apparent molecular weight 14kDa, 15kDa, 20kDa and 22kDa.

Keywords: Fn2 type proteins, Buck seminal plasma, Affinity chromatography

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