JVAS announces awards - Four best research articles (one each from basic, production, para-clinical and clinical subjects) and one best short communication will be adjudged for awards each year!!!

Journal of Veterinary and Animal Sciences

Volume: 54 Issue: 3

  • Open Access
  • Research Article

Screening of beef and raw cow milk from central Kerala for Campylobacter spp. using multiplex PCR

Devika D. Sunil1, Binsy Mathew1*, C. Latha1, Deepa Jolly1 and K. Radha2

1. Department of Veterinary Public Health

2. Department of Dairy Sciences and Technology

College of Veterinary and Animal Sciences, Mannuthy, Thrissur- 680 651, Kerala Veterinary and Animal Sciences University Kerala, India

*Corresponding author: [email protected] Ph.9072263357

Year: 2023, Page: 636-640, Doi: https://doi.org/10.51966/jvas.2023.54.3.636-640

Received: Nov. 14, 2022 Accepted: Jan. 13, 2023 Published: Sept. 30, 2023

Abstract

Campylobacter spp. is considered one of the leading causes of diarrhoeal disease. Due to the prevalence of this organism in the gut microbiota of all warm-blooded animals, the chance of contamination is more through animal products. The present study was conducted to assess the occurrence of Campylobacter spp. in milk and beef of central Kerala. A total of 200 beef and 200 raw cow milk samples were collected from various retail outlets and milk societies of Thrissur and Ernakulam districts. Campylobacter spp. were isolated by conventional culture technique on Modified charcoal-cefoperazone-deoxycholate agar. A molecular technique targeting the genusspecific 16S rRNA gene further confirmed the culture and biochemically positive isolates. The species-specific mapA and ceuE genes were targeted for C. jejuni and C. coli, respectively. None of the milk samples collected were positive for Campylobacter spp. However, three per cent of beef samples were tested positive for Campylobacter spp.

Keywords: Campylobacter spp., milk, beef

References

Abri, R., Javadi, A., Asghari, R., Razavilar, V., Salehi, T.Z., Safaeeyan, F. and Rezaee, M.A. 2019. Surveillance for enterotoxigenic & enteropathogenic Escherichia coli isolates from animal source foods in Northwest Iran. Ind J. Med. Res.150: 87p.

Athulya, T.R., Latha, C., Sunil, B., Deepa, J. and Shynu, M. 2021. Occurrence of Campylobacter spp. in duck and associated environmental samples in Thrissur district. J. Vet. Anim. Sci. 52: 325-330.

Denis, M., Soumet, C., Rivoal, K., Ermel, G., Blivet, D., Salvat, G. and Colin, P. 1999. Development of am‐PCR assay for simultaneous identification of Campylobacter jejuni and C. coli. Lett. Appl. Microbiol. 29:406-410.

Du, Y., Wang, C., Ye, Y., Liu, Y., Wang, A., Li, Y., Zhou, X., Pan, H., Zhang, J. and Xu, X. 2018. Molecular identification of multidrug-resistant Campylobacter spp. from diarrheal patients and poultry meat in Shanghai, China. Frnt.  Microbiol. 9: 16-42.

Hagos, Y., Gugsa, G., Awol, N., Ahmed, M., Tsegaye, Y., Abebe, N. and Bsrat, A. 2021. Isolation, identification, and antimicrobial susceptibility pattern of Campylobacter jejuni and Campylobacter coli from cattle, goat, and chicken meats in Mekelle, Ethiopia. PloS one16: 20p.

Hansson, I., Vagsholm, I., Svensson, L., Olsson, E. 2007. Correlations between Campylobacter spp. prevalence in the environment and broiler flocks. J. Appl. Microbiol. 103: 640-649.

Igwaran, Aboi, and Anthony I. Okoh. 2020. Campylobacteriosis agents in meat carcasses collected from two district municipalities in the Eastern Cape Province, South Africa. Foods 2: 203.

Joby, J.E. 2016. Occurrence of Campylobacter spp. in chicken egg production chain. M.V.Sc thesis, Kerala Veterinary and Animal Sciences University, Pookode, 64p.

Khan, J.A., Rathore, R.S., Abulreesh, H.H., Qais, F.A. and Ahmad, I. 2018. Prevalence and antibiotic resistance profiles of Campylobacter jejuni isolated from poultry meat and related samples at retail shops in Northern India. Foodborne Pathog. Dis. 15: 218-225.

Korsak, D., Mackiw, E., Rozynek, E., Zyłowska, M. 2015. Prevalence of Campylobacter spp. in retail chicken, turkey, pork, and beef meat in Poland between 2009 and 2013. J. Food Prot. 78: 1024-1028.

Linton, D., Owen, R.J. and Stanley, J. 1996. Rapid identification by PCR of the genus Campylobacter and of five Campylobacter species enteropathogenic for man and animals. Res. Microbiol. 147: 707-718.

Modi, S., Brahmbhatt, M.N., Chatur, Y.A. and Nayak, J.B. 2015. Prevalence of Campylobacter species in milk and milk products, their virulence gene profile and anti-bio gram. Vet. Wld. 8: 2p.

Narvaez-Bravo, C., Taboada, E.N., Mutschall, S.K. and Aslam, M. 2017. Epidemiology of antimicrobial resistant Campylobacter spp. isolated from retail meats in Canada. Int. J. Food Microbiol. 253:43-47.

OIE. [Office International des Epizooties]. 2017. Infection with C. coli and C. jejuni. [Chapter 2.9.3.]. OIE Terrestrial Manual. World Organisation for Animal Health, 9p.

Singh, R., Singh, P.P., Rathore, R.S., Dhama, K. and Malik, S.V.S. 2009. Prevalence of Campylobacter jejuni and Campylobacter coli in meat and carcasses collected from local poultry farms and retail shops of Bareilly, Uttar Pradesh, India. Indian J. Comp. Microbiol. Immunol. Infect. Dis30: 35-58.

Swetha, S.V., Babu, A.J., Rao, T.M. and Kumar, E. 2015. Evaluation of various selective and non-selective broths for detection of Listeria monocytogenes in pork and for PCR compactibility. Int. J. Adv. Res. 3: 316-327.

Vani, R.P., 2018. Identification of Campylobacter spp. critical control points in beef production chain. M. V. Sc. thesis, Kerala Veterinary and Animal Sciences University, Pookode, 96p.

Vivekanandhan, R., Sunil, B., Latha, C., Vrinda Menon, K., Ambily, R. and Gleeja, V.L. 2022. Occurrence of thermophilic Campylobacter spp. in pigs and the assessment of biosecurity measures employed at unorganized pig farms in Thrissur, Kerala. J. Vet. Anim. Sci. 53: 602-608.

Wegmuller, B., Luthy, J. and Candrian, U. 2003. Direct polymerase chain reaction detection of Campylobacter jejuni and Campylobacter coli in raw milk and dairy products. Appl. Environ. Microbiol. 59: 2161-2165.

WHO. 2020. WHO estimates of the global burden of foodborne diseases: foodborne disease burden epidemiology reference group 2016-2020.World Health Organization.

Zang, X., Kong, K., Tang, H., Tang, Y., Tang, H., Jiao, X. 2018. A GICA strip for Campylobacter jejuni real-time monitoring at meat production site. Vet. Wld. 98:500- 505.

Cite this article

Devika D.S., Mathew B., Latha C., Jolly D. and Radha K. 2023. Screening of beef and raw cow milk from central Kerala for Campylobacter spp. using multiplex PCR. J. Vet. Anim. Sci. 54(3): 636-640

DOI: https://doi.org/10.51966/jvas.2023.54.3.636-640

Views
246
Downloads
139
Citations