Volume: 57 Issue: 3
Year: 2026, Page: 491-493, Doi: https://doi.org/10.51966/jvas.2026.57.3.491-493
Received: April 27, 2026 Accepted: June 18, 2026 Published: Sept. 30, 2026
Newcastle disease (ND), caused by Newcastle disease virus (NDV), is a major viral disease affecting poultry production worldwide. The present study aimed to analyse the comparative expression of prostaglandin-endoperoxide synthase 2 (PTGS2) in embryonated chicken eggs of Gramasree and Tellicherry breeds at different stages of embryonic development following NDV infection. A lentogenic F strain NDV vaccine of infectivity titre of 2.34 × 10⁷ EID₅₀/mL was used for experimental infection. Embryonated eggs at 12, 15 and 18 days of incubation were inoculated along with age matched controls. Total RNA extracted from embryonic tissues was subjected to cDNA synthesis followed by quantitative real-time PCR analysis. The results demonstrated upregulation of PTGS2 expression following NDV infection, with expression remaining elevated at the later developmental stages. Comparative analysis revealed higher fold changes in Tellicherry embryos than in Gramasree, indicating breed-dependent variation in the inflammatory response to NDV infection.
Keywords: Newcastle disease virus; PTGS2; embryonated chicken eggs gene expression; Tellicherry; Gramasree
Deist, M. S., Gallardo, R. A., Bunn, D. A., Kelly, T. R., Dekkers, J. C., Zhou, H., & Lamont, S. J. (2018). Novel analysis of the Harderian gland transcriptome response to Newcastle disease virus in two inbred chicken lines. Scientific Reports, 8(1), 6558.10.1038/s41598-018-24830-0
Dimitrov, K. M., Lee, D. H., Williams-Coplin, D., Olivier, T. L., Miller, P. J., & Afonso, C. L. (2016). Newcastle disease viruses causing recent outbreaks worldwide show unexpectedly high genetic similarity to historical virulent isolates from the 1940s. Journal of Clinical Microbiology, 54(5), 1228–1235.10.1128/ JCM.03044-15
Kapczynski, D. R., Afonso, C. L., & Miller, P. J. (2013). Immune responses of poultry to Newcastle disease virus. Developmental & Comparative Immunology, 41(3), 447–453.10.1016/j.dci.2013.04.012
Karpala, A. J., Stewart, C., McKay, J., Lowenthal, J. W., & Bean, A. G. (2011). Characterization of chicken MDA5 activity: Regulation of IFN-β in the absence of RIG-I functionality. Journal of Immunology, 186(9), 5397–5405. https://doi.org/10.4049/jimmunol.1003712
Miller, P. J., & Koch, G. (2013). Newcastle disease. In D. E. Swayne, J. R. Glisson, L. R. McDougald, L. K. Nolan, D. L. Suarez, & V. L. Nair (Eds.), Diseases of poultry (13th ed., pp. 89–107). Wiley-Blackwell.
Rue, C. A., Susta, L., Cornax, I., Brown, C. C., Kapczynski, D. R., Suarez, D. L., King, D. J., Miller, P. J., & Afonso, C. L. (2011). Virulent Newcastle disease virus elicits a strong innate immune response in chickens. Journal of General Virology, 92(4), 931–939. https:// doi.org/10.1099/vir.0.025486-0
Takeuchi, O., & Akira, S. (2009). Innate immunity to virus infection. Immunological Reviews, 227(1), 75–86. https://doi.org/10.1111/j.1600-065X.2008.00737
Wang, C., Wang, T., Hu, R., Dai, J., Liu, H., Li, N., Schneider, U., Yang, Z., & Wang, J. (2020). Cyclooxygenase-2 facilitates Newcastle disease virus proliferation and is a target for canthin-6-one antiviral activity. Frontiers in Microbiology, 11, 987. https://doi. org/10.3389/fmicb.2020.00987
© 2026 Sajna et al. This is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Sajna, S., Nair, S. R., Priya, P. M., Surya, S., Ambily, R., Elizabeth, K. 2026. Comparative expression analysis of PTGS2 in Gramasree and Tellicherry breeds of chicken across different embryonic stages. Journal of Veterinary and Animal Sciences, 57(3), 491-493