Volume: 40 Issue: 2
Year: 2009, Page: 35-36, Doi: https://doi.org/10.51966/jvas.2009.40.35-36
Received: April 13, 2009 Accepted: Aug. 13, 2009 Published: Dec. 31, 2009
Comparative efficacy of acid fast staining of faecal smear, single intradermal Johnin test and IS900 faecal PCR was investigated in fifty goats for detection of Mycobacterium avium subspecies paratuberculosis (MAP). Out of the fifty goats screened for paratuberculosis from field, one goat (2 per cent), three goats (6 per cent) and twelve goats (24 per cent) were found positive for the organism by Ziehl-Neelsen acid fast staining of faecal smear, single intradermal Johnin test and by IS900 PCR respectively. Results of present study indicate that amplification of the IS900 insertion element was the most suitable diagnostic detection method analysed in this study. The strategic use of PCR can provide a means for early identification of MAP infected goat and thus ensuring their removal from an infected herd.
Keywords: Caprine paratuberculosis, faecal PCR