Journal of Veterinary and Animal Sciences

Volume: 55 Issue: 2

  • Open Access
  • Research Article

Development of folate enriched yoghurt incorporating Lacticaseibacillus paracasei

A.S. Lejin1, R. Geetha1*, C.T. Sathian1, Magna Thomas2, M.V. Chinnu3 and R. Rejeesh4

1. Department of Dairy Sciences College of Veterinary and Animal Science, Mannuthy, Thrissur - 680 651 Kerala Veterinary and Animal Sciences University Kerala, India

2. Department of Dairy Science, CVAS, Pookode

3. Department of Veterinary Biochemistry

4. Department of Dairy Microbiology, VKIDFT, Mannuthy

*Corresponding author: [email protected] , Ph. 9495289976

Year: 2024, Page: 240-246, Doi: https://doi.org/10.51966/jvas.2024.55.2.240-246

Received: Jan. 1, 2024 Accepted: Feb. 22, 2024 Published: June 30, 2024

Abstract

The aim of the study was to develop folate-enriched yoghurt incorporating lactic acid bacterial isolate obtained from natural sources. Ten lactic acid bacterial isolates obtained from natural sources, available in the Department of Dairy Science, College of Veterinary and Animal Sciences, Mannuthy, were screened to assess their potential for folate production. Optimisation studies were designed at two varying temperatures (37°C and 42°C) and three fermentation intervals (4h, 8h and 12h). In the study isolate “L1” was identified as a superior folate producer (17.67±1.78 µg/L) as enhanced folate production was achieved when grown at a temperature of 42°C for a period of 4 h which is considered as ideal fermentation condition for yoghurt preparation. Lactobacillus rhamnosus (NCDC 18), the standard strain yielded 11.38±0.91 µg/L. Molecular characterisation revealed that the isolate “L1” is Lacticaseibacillus paracasei. Functional yoghurt (treatment T) was formulated incorporating L. paracasei as an adjunct culture along with yoghurt cultures. Control 1 (C1) was prepared using standard yoghurt cultures, while control 2 (C2) was supplemented with L. rhamnosus as an adjunct culture in addition to the standard yoghurt cultures. A significantly higher (p<0.05) folate content was observed in functional yoghurt sample (T), with a value of 105.72±15.48 µg/L, when compared to control. In the sensory evaluation of yoghurt samples, a significantly higher overall score was observed for sample T. This suggests that the addition of L. paracasei can be a viable approach for developing folate-enriched yoghurt with acceptable sensory quality Notably, folate production ability of isolate “L6” (224±36.93 µg/L over a 12h period at 37°C) can also be exploited for increasing folate level in other fermented dairy products, by way of natural synthesis.

Keywords: folate, yoghurt, Lacticaseibacillus paracaei

References

Chaitanya, R.K., Priyanka, G. and Sreedevi, B. 2021. Evaluation of real time PCR for the detection of Mycobacterium avium subsp. paratuberculosis in faecal samples of cattle. J. Vet. Anim. Sci. 52: 414-417.

Crittenden, R. G., Martinez, N. R. and Playne, M. J. 2003. Synthesis and utilisation of folate by yoghurt starter cultures and probiotic bacteria. Int. J. Food Microbiol. 80: 217–222.

Horne, D. W. and Patterson, D. 1988. Lactobacillus casei microbiological assay of folic acid derivatives in 96-well microtiter plates. Clin. Chem. 34: 2357– 2359.

Iyer, R. and Tomar, S.K. 2009. Folate: a functional food constituent. J. Food Sci. 74: 114-122.

Kneifel, W., Holub, S. and Wirthmann M. 1989. Monitoring of B-complex vitamins in yoghurt during fermentation. J Dairy Res. 56: 651–656.

Laino, J.E., del Valle, M.J., de Giori, G.S. and LeBlanc, J.G.J., 2013. Development of a high folate concentration yoghurt naturally bio-enriched using selected lactic acid bacteria. LWT - Food Sci. Technol. 54: 1-5.

Laino, J. E., LeBlanc, J. G. and de Giori, G. S. 2012. Production of natural folates by lactic acid bacteria starter cultures isolated from artisanal argentinean yoghurts. Can. J. Microbiol. 58: 581– 588.

Larmond, E. 1977. Laboratory methods for sensory evaluation of food. Food Sci. Technol. 95: 315-321.

Morgan, M.C., Boyette, M., Goforth, C., Sperry, K.V. and Greene, S.R. 2009. Comparison of the Biolog OmniLog Identification System and 16S ribosomal RNA gene sequencing for accuracy in identification of atypical bacteria of clinical origin. J. Microbiol. Methods. 79: 336–343.

Mosso, A.L., LeBlanc, J.G., Motta, C., Castanheira, I., Ribotta, P. and Samman, N. 2020. Effect of fermentation in nutritional, textural and sensorial parameters of vegan-spread products using a probiotic folate-producing Lactobacillus sakei strain. LWT. 127: 109339.

Patil, M.M., Pal, A., Anand, T. and Ramana, K.V. 2010. Isolation and characterization of lactic acid bacteria from curd and cucumber.Indian J. Biotechnol. 9: 166– 172.

Rekha, R.S., Radha, K. and Sathian, C.T. 2018. Development and quality studies of folate enriched yoghurt by incorporating folate producing bacteria. Ind. J. Dairy Sci. 71: 41-46.

Sybesma, W., Starrenburg, M., Kleerebezem, M., Mierau, I., de Vos, W.M. and Hugenholtz, J. 2003. Increased production of folate by metabolic engineering of Lactococcus lactis. Appl. Environ. Microbiol. 69: 3069-3076.

Tarakci, Z. and Kucukoner, E. 2003. Physical, chemical, microbiological and sensory characteristics of some fruit-flavored yoghurt. YYU Vet Fak Derg. 14: 10-14.

Vanitha, H.D., Sethulekshmi, C., Latha, C., Prejit, R. and Mercey, K.A. 2018. Molecular detection of enterohaemorrhagic Escherichia coli in raw milk samples of Thrissur. J. Vet. Anim. Sci. 1: 44-47.

Wouters, J.T., Ayad, E.H., Hugenholtz, J. and Smit, G. 2002. Microbes from raw milk for fermented dairy products. Int. Dairy J. 12: 91-109.

Cite this article

Lejin, A. S., Geetha, R., Sathian, C. T., Magna, T., Chinnu, M.V. and Rejeesh, R. 2024. Development of folate enriched yoghurt incorporating Lacticaseibacillus paracasei. J. Vet. Anim. Sci. 55(2):240-246

DOI: https://doi.org/10.51966/jvas.2024.55.2.240-246

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