Volume: 54 Issue: 1
Year: 2023, Page: 174-179, Doi: https://doi.org/10.51966/jvas.2023.54.1.174-179
Received: Nov. 3, 2022 Accepted: Dec. 20, 2022 Published: March 31, 2023
A feeding trial was conducted for a period of 120 days for studying the haemato-biochemical and sex hormonal parameters in buffalo heifers fed with concentrate mixtures containing varying levels of dried moringa (Moringa oleifera) leaves. Fifteen Murrah buffalo heifers aged 18-26 months were selected and randomly allotted to three groups of five animals each and fed three dietary treatments, containing concentrate mixture (20 per cent CP and 68 per cent TDN) and ad libitum green grass. Dried moringa leaves were incorporated at 0, 10 and 20 per cent in the concentrate mixture for treatments T1 (control), T2 and T3 respectively. Comparison of serum biochemical values revealed higher serum glucose and oestrogen in buffalo heifers fed T3 when compared to those fed T1 and T2. The serum cholesterol values were significantly lower (p<0.05) for buffalo heifers fed T2 and T3 when compared to T1. Evaluation of the results obtained in the present study revealed that moringa leaves can be effectively incorporated up to 20 per cent in the concentrate mixture of buffalo heifers without any adverse effects on haemato-biochemical parameters with improved oestrogen levels indicating better reproductive performance.
Keywords: Murrah buffalo heifers, dried moringa leaves, haemato-biochemical parameters, sex hormones
Abd-Ellah, M.R., Hamed, M.I., Ibrahim, D.R. and Rateb, H.Z. 2014. Serum biochemical and haematological reference intervals for water buffalo (Bubalus bubalis) heifers. J. S. Afr. Vet. Ass. 85: 1-7.
Abdel-Raheem, S.M. and Hassan, E.H. 2021. Effects of dietary inclusion of Moringa oleifera leaf meal on nutrient digestibility, rumen fermentation, ruminal enzyme activities and growth performance of buffalo calves. Saudi J. Biol. Sci. 28: 4430-4436.
Abdoon, A.S., Attia, M.Z., Soliman, S.S., Kandil, O.M., El-Toukhey, N.E. and Sabra, H.A. 2020. Seasonal variation in number of ovarian follicles and hormonal levels in Egyptian Buffalo and cattle. Int. J. Vet. Sci. 9: 126-130.
Aharwal, B., Roy, B., Lakhani, G. P. and Yadav, A. 2019. Effect of Moringa (Moringa oleifera) leaf meal on performance of Murrah buffalo calves. Intl. J. Chem. Stud. 7: 1722-1728.
Ahmed W.M., El-khadrawy H.H., Ali A.H. and Shalaby S.A. 2010. Clinical perspective of repeat breeding syndrome in buffaloes. J. Am. Sci. 6: 661-666.
AOAC [Association of Official Analytical Chemists]. 2016. Official Methods of Analysis. (20th Ed.). Association of Official Analytical Chemists, Washington DC, 1885p.
Dhakad, G.S., Aich, R., Sharda, R. and Jain, R.K. 2016. Estradiol and progesterone levels during pregnancy in Murrah buffaloes. Indian J. Vet. Sci. Biotech. 11: 38-39.
Djais, A.I., Thahir, H., Hatta, M., Achmad, H. and Sari, M. 2019. Effect of moringa leaf extract (Moringa oleifera) on increasing the number of osteoblast as a marker of bone remodeling. Indian J. Public Hlth. Res. Dev. 10:1394-1398.
Dutta, P., Harikumar, A.V., Shroff, S.I., Rana, S.K., Mogale, U.V., Magare, V., Punniamurthy, N., Nair, M.N.B. and Gorhe, S.B. 2022. Management of repeat breeding in bovine by herbal combination. J. Ent. Zool. Stud. 10: 295-297.
Elaidy, A.A., Abou Selim, I.A., Abou-Elenin, E.I., Abbas, M.S. and Sobhy, H.M. 2017. Effect of feeding dry Moringa oleifera leaves on the performance of suckling buffalo calves. Asian J. Anim. Sci. 11: 32-39.
Gupta, K., Barat, G.K., Wagle, D.S. and Chawla, H.K.L. 1989. Nutrient contents and antinutritional factors in conventional and non-conventional leafy vegetables. Food chem. 31:105-116.
ICAR [Indian Council of Agricultural Research]. 2013. Nutrient Requirements of Animals-Cattle and Buffalo. (3rd Ed.). Indian Council of Agricultural Research, New Delhi, 59p.
Jong, H.H. and Vegeter, J.J. 1950. Determination of serum proteins with the biuret test. Pharm. Weekbl. 85: 755-764.
Prasad, B.C., Srinivas, M. and Thangamani, A. 2020. Herbs as nutrients in true anestrous buffaloes and its comparative evaluation. Intas Polivet. 21: 406-408.
Priya, P., Ajith, K.S., Ally, K., Shyama, K. and Venkatachalapathy, T.R. 2020. Effect of complete feed containing Moringa oleifera and Sorghum sudanense on milk yield of lactacting Malabari goats. J. Vet. Anim. Sci. 51: 65-69.
Rema, L.P. and Liji, C.B. 2021. Insights into the role of Moringa oleifera in ameliorating acetaminophen induced hepatotoxicity in Nile tilapia, Oreochromis niloticus. J. Vet. Anim. Sci. 52: 399-404.
Sánchez-Machado, D.I., Núñez-Gastélum, J.A., Reyes-Moreno, C., Ramírez-Wong, B. and López-Cervantes, J. 2010. Nutritional quality of edible parts of Moringa oleifera. Food Analyt. Methods. 3: 175-180.
Sánchez, N.R., Ledin, S. and Ledin, I. 2006. Biomass production and chemical composition of Moringa oleifera under different management regimes in Nicaragua. Agrofor. Syst. 66: 231-242.
Kumar, S.S., Punniamurthy, N. and Ranganathan, V. 2021. Herbal Combo therapy for oestrus induction in postpartum anoestrus cows. J Phytopharmacol. 10: 19-21.
Sharma, S., Nigam, R., Pandey, V., Ghuman, S.S. and Singh, P. 2015. Circulating levels of estradiol 17-β and progesterone vis à vis nitric oxide and nitric oxide synthase at and around oestrus in cycling buffaloes. J. Appl. Anim. Res. 43: 214-217.
Snedecor, G.W. and Cochran, W.G. 2014. Statistical Methods. (8th Ed.). The Iowa State University press, Ames, Iowa, USA. 503p.
Takkar, O.P., Singh, M. and Varman, P.N. 1982. Progesterone profile in buffaloes during various stages of oestrous cycle using radioimmunoassay technique. Theriogenology. 17: 565-569.
Wafa, W.M., El-Nagar, H.A., Gabr, A.A. and Rezk, M.M. 2017. Impact of dietary Moringa oleifera leaves supplementation on semen characteristics, oxidative stress, physiological response and blood parameters of heat stressed buffalo bulls. J. Anim. Poult. Prod. Mansoura Univ. 8: 367-379.
© 2023 Margret et al. This is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Margret,T., Syam Mohan,K.M., Ally,K., George,S.K. and Anil,K.S. 2023. Effect of feeding concentrate mixtures containing varying levels of dried moringa (Moringa oleifera) leaves on haemato-biochemical and sex hormonal parameters in Murrah buffalo heifers. J. Vet. Anim. Sci. 54(1):174-179
DOI: https://doi.org/10.51966/jvas.2023.54.1.174-179