Volume: 54 Issue: 2
Year: 2023, Page: 396-403, Doi: https://doi.org/10.51966/jvas.2023.54.2.396-403
Received: Nov. 9, 2022 Accepted: March 7, 2023 Published: June 30, 2023
A study was conducted on 96 Vencobb 430, day-old broiler chicks to evaluate the effect of steam treatment on banana inflorescence supplementation as prebiotic on intestinal morphology. The birds were randomly assigned into three treatment groups having four replicates with eight birds per replicate and assigned control diet, formulated in accordance with BIS recommendation to control (T1) group and with supplementation of 0.5% dried banana inflorescence (T2 group) or 0.5% steam treated banana inflorescence (T3 group). After 42 days of feeding trial, the live weight was significantly higher in T3 and T2 than control. Two birds from each replicate were sacrificed to study the intestinal morphometry and pH. The weight of intestine varied significantly (p<0.01) between the treatments and was higher in T3 while the per cent of intestinal weight did not differ significantly. Intestinal morphometry revealed that the villus height was significantly (p<0.01) higher in duodenum, jejunum, and caecum in T3 and ileum in T2. The crypt depth was significantly (p<0.01) higher in jejunum, ileum and caeca in T3 and T2 in duodenum. Caecal crypt depth showed significantly (p< 0.01) low value in T2. Ileal crypt depth ratio was significantly (p<0.01) higher in T2 while the caecum crypt depth was significantly(p<0.01)
Keywords: Banana inflorescence, Lactobacillus, histomorphometry
Al-Baadani, H.H., Abudabos, A.M., Al-Mufarrej, S.I. and Alzawqari, M. 2016. Effects of dietary inclusion of probiotics, prebiotics and synbiotics on intestinal histological changes in challenged broiler chickens. S. Afr. J. Anim. Sci. 46:157-165.
Baurhoo, B., Phillip, L. and Ruiz-Feria, C.A. 2007. Effects of purified lignin and mannan oligosaccharides on intestinal integrity and microbial populations in the ceca and litter of broiler chickens. Poult. Sci. 86: 1070-1078.
Eyng, C., Murakami, A.E., Duarte, C.R.A. and Santos, T.C. 2014. Effect of dietary supplementation with an ethanolic extract of propolis on broiler intestinal morphology and digestive enzyme activity. J. Anim. Physiol. Anim. Nutr. 98: 393-401.
Fingolo, C.E., Braga, J., Vieira, A., Moura, M.R. and Kaplan, M.A.C. 2012. The natural impact of banana inflorescences (Musa acuminata) on human nutrition. An. Acad. Bras. Cienc. 84: 891-898
Houshmand, M., Azhar, K., Zulkifli, I., Bejo, M.H. and Kamyab, A. 2011. Effects of nonantibiotic feed additives on performance, nutrient retention, gut pH, and intestinal morphology of broilers fed different levels of energy. J. Appl. Poult. Res. 20: 121-128.
Lau, B.F., Kong, K.W., Leong, K.H., Sun, J., He, X., Wang, Z., Mustafa, M.R., Ling, T.C., Ismail, A. (2020). Banana inflorescence: Its bio-prospects as an ingredient for functional foods. Trends. Food Sci. Technol. 97: 14-28.
Luna, L.G. 1968. Manual of histologic staining methods of the Armed Forces Institute of Pathology. (3rd Ed). McGraw-Hill, New York.
Iji, P.A. and Tivey, D.R. 1998. Natural and synthetic oligosaccharides in broiler chicken diets. Worlds Poult. Sci. J. 54: 129-143.
Izadi, H., Arshami, J., Golian, A. and Reza Raji, M. 2013. Effects of chicory root powder on growth performance and histomorphometry of jejunum in broiler chicks. Vet. Res. Forum. 4: 169–174
Nguyen, D.T., Le, N.H.,Pham, V.V., Eva, P., Alberto, F. and Le, H.T., Relationship between ratio of villous height: crypt depth and gut bacteria counts as well as production paarameters in broiler chickens. J. Agric. Dev. 20: 1-10.
Pelicano, E.R.L., Souza, P.D., Souza, H.D., Figueiredo, D.F., Boiago, M.M., Carvalho, S.R. and Bordon, V.F. 2005. Intestinal mucosa development in broiler chickens fed natural growth promoters. Brazilian J. Poult. Sci. 7: 221-229.
Perrier, X., De Langhe, E., Donohue, M., Lentfer, C., Vrydaghs, L., Bakry, F., Carreel, F., Hippolyte, I., Horry, J.P., Jenny, C. and Lebot, V. 2011. Multidisciplinary perspectives on banana (Musa spp.) domestication. Proc. Nati. Acad. Sci. 108: 11311-11318
Pushpaveni, C., Visagaperumal D. and C. Vineeth. 2019. A Review on Banana Blossom: The Heart of Banana. J. Pharm. Pharmacogn. Res. 8: 440-450.
Santin, E., Maiorka, A., Macari, M., Grecco, M., Sanchez, J.C., Okada, T.M. and Myasaka, A.M. 2001. Performance and intestinal mucosa development of broiler chickens fed diets containing Saccharomyces cerevisiae cell wall. J. Appl. Poul. Res. 10: 236-244
Shajini, S., Beena C. Joseph, Anitha, P., Harikrishnan S. and Sajith Purushothaman. 2022. Effect of chitosan and L-carnitine supplementation on carcass characteristics of broiler chicken. J. Vet. Anim. Sci. 53: 413-416
Suffi, N.S.M., Mohamed, E., Camalxaman, S.N., Rambely, A.S. and Haron, N. 2021. The medicinal benefits, phytochemical constituents and antioxidant properties of banana blossom: A mini review. Healthscope. 4: 113-118.
Supriya, R., Chacko, B., Anitha, P., Suja, C. S. and Priya, P. M. 2022. Effects of fermented soybean meal, probiotics and organic acids on intestinal morphology, pH and microbial count in broilers. J. Vet. Anim. Sci. 53: 348-355.
Tsirtsikos, P., Fegeros, K., Balaskas, C., Kominakis, A. and Mountzouris, K.C., 2012. Dietary probiotic inclusion level modulates intestinal mucin composition and mucosal morphology in broilers. Poult. Sci. 91:1860-1868.
Wang, X., Farnell, Y.Z., Peebles, E.D., Kiess, A.S., Wamsley, K.G.S. and Zhai, W. 2016. Effects of prebiotics, probiotics, and their combination on growth performance, small intestine morphology, and resident Lactobacillus of male broilers. Poult Sci. 95: 1332-1340.
Waqas, M., Mehmood, S., Mahmud, A., Hussain, J., Ahmad, S., Khan, M.T., Rehman, A., Zia, M.W. and Shaheen, M.S. 2019. Effect of yeast based mannan oligosaccharide (Actigen™) supplementation on growth, carcass characteristics and physiological response in broiler chickens. Indian J. Anim. Res. 53: 1475-1479.
© 2023 Ambili et al. This is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Ambili, K., Deepa, A., Biju C., Senthil M.S., Patki, H.S., Surjith, K.P., Muneer, A. and Dinsha, T.K. 2023. Effect of supplementation of differentially treated banana inflorescence on intestinal morphology in broiler birds. J. Vet. Anim. Sci. 54(2):396-403
DOI: https://doi.org/10.51966/jvas.2023.54.2.396-403