Volume: 53 Issue: 3
Year: 2022, Page: 471-477, Doi: https://doi.org/10.51966/jvas.2022.53.3.471-477
Received: March 19, 2022 Accepted: June 9, 2022 Published: Sept. 30, 2022
A study was conducted to examine the effect of supplementation of exogenous fibrolytic enzymes (EFE) carboxymethylcellulase (CMC) and xylanase (Xy) on milk yield and composition in early lactating crossbred dairy cows fed on total mixed ration (TMR). Fifteen cows within ten days of calving were selected from University Livestock Farm and Fodder Research and Development Scheme (ULF & FRDS), Mannuthy and were randomly divided into three groups. The animals were fed with isonitrogenous TMR (3.5 per cent of body weight) for a period of 90 days and the dietary treatments included; T1 offered with the control diet (TDN - 60 per cent, CP - 13 per cent), T2 offered with control diet supplemented with EFE (CMC 3500 units/kg and Xy 16000 units/kg), and T3 given a diet lower in TDN (TDN - 55 per cent) supplemented with EFE (CMC 3500 units/kg and Xy 16000 units/kg).The results of the study revealed that milk yield remained unaffected (p>0.05) with dietary treatments. Milk composition also remained similar, except for milk urea nitrogen which was lower (p<0.05) in enzyme supplemented groups. Thus, it could be inferred that EFE supplementation in cows during early lactation is more beneficial in diets with low TDN since the milk yield and milk composition of cows fed with TMR having low TDN supplemented with EFE had comparable values with those offered with TMR having higher TDN.
Keywords: Exogenous fibrolytic enzymes, total mixed ration, early lactating dairy cows
AOAC [Association of Official Analytical Chemists]. 2016. Official Methods of Analysis.(20th Ed.). Association of Official Analytical Chemists, Washington D.C., 1885p.
Arif, M., Al-Sagheer, A.A., Salem, A.Z.M., Abd El-Hack, M.E., Swelum, A.A., Saeed, M., Jamal, M. and Akhtar, M. 2019. Influence of exogenous fibrolytic enzymes on milk production efficiency and nutrient utilization in early lactating buffaloes fed diets with two proportions of oat silage to concentrate ratios. Livestock Sci. 219: 29-34.
Beauchemin, K.A., Colombatto, D., Morgavi, D.P. and Yang, W.Z. 2003. Use of exogenous fibrolytic enzymes to improve feed utilization by ruminants. J. Anim. Sci. 81: E37-E47.
Bector, B.S., Ram, M. and Singhal, O.P. 1998. Rapid platform test for detection and determination of added urea in milk. Indian Dairyman. 50: 59-62.
Chacko, B., Mohan, K.M.S., Ally, K., Shyama, K., Anil, K.S. and Sathian, C.T. 2016. Production performance of cows reared on complete feeds with varying levels of neutral detergent fibre (NDF) as against the conventional grass concentrate feeding system in Kerala. J. Vet. Anim. Sci. 47(1): 36-41.
El-Bordeny, N.E., Abedo, A.A., El-Sayed, H.M., Daoud, E.N., Soliman, H.S. and Mahmoud, A.E.M. 2015.Effect of exogenous fibrolytic enzyme application on productive response of dairy cows at different lactation stages. Asian J. Anim. Vet. Adv. 10: 226-236.
IS: 1224.[Indian Standards Institution]. 1977. Determination of fat by Gerber’s Method part 1. Milk (First Revision), Indian Standards Institution, New Delhi, 10p.
Jayaraj, N., Purushothaman, S., Ally, K., Ananth, D. and Simon, S. 2021. Effect of supplementation of rumen protected choline and methionine on milk yield and composition of early lactating dairy cows. J. Vet. Anim. Sci. 52(2): 142-148.
Knowlton, K.F., Taylor, M.S., Hill, S.R., Cobb, C. and Wilson, K.F. 2007. Manure nutrient excretion by lactating cows fed exogenous phytase and cellulase. J. Dairy Sci. 90: 4356-4360.
Krueger, N.A., Adesogan, A.T., Staples, C.R., Krueger, W.K., Kim, S.C., Littell, R.C. and Sollenberger, L.E. 2008. Effect of method of applying fibrolytic enzymes or ammonia to Bermudagrass hay on feed intake, digestion, and growth of beef steers. J. Anim. Sci. 86: 882-889.
Łopuszańska-Rusek, M. and Bilik, K. 2011. Influence of pre-and postpartum supplementation of fibrolytic enzymes and yeast culture, or both, on performance and metabolic status of dairy cows. Ann. Anim. Sci. 11: 531-545.
Lunsin, R., Pilajun, R., Cherdthong, A., Wanapat, M., Duanyai, S. and Sombatsri, P. 2021. Influence of fibrolytic enzymes in total mixed ration containing urea‐molasses‐treated sugarcane bagasse on the performance of lactating Holstein–Friesian crossbred cows. Anim. Sci. J. 92: e13652.
Miachieo, K. and Thakur, S.S. 2007. Effect of exogenous fibrolytic enzymes on the productive performance of lactating Sahiwal cows. Indian J. Anim. Nutr. 24: 27-30.
NRC [National Research Council].2001. Nutrient requirements for dairy cattle. (7th Ed.). National Academy Press, Washington D.C., U.S.A., 408p.
Peters, A., Meyer, U. and Dänicke, S. 2015. Effect of exogenous fibrolytic enzymes on performance and blood profile in early and mid-lactation Holstein cows. Anim. Nutr. 1: 229-238.
Romero, J.J., Macias, E.G., Ma, Z.X., Martins, R.M., Staples, C.R., Beauchemin, K.A. and Adesogan, A.T. 2016.Improving the performance of dairy cattle with a xylanase-rich exogenous enzyme preparation. Dairy Sci. 99: 3486-3496.
Shadmanesh, A. 2014.Effect of dietary supplement with fibrolytic enzymes on the productive performance of early lactating dairy cows. Indian J. Fundamental Appl. Life Sci. 4: 396-401.
Snedecor, G.W. and Cochran, W.G. 2014. Statistical Methods (8th Ed.). The Iowa state university press, Ames, Iowa, USA. 503p.
Sticklen, M.B. 2008. Plant genetic engineering for biofuel production: towards affordable cellulosic ethanol. Nat. Rev. Genet. 9: 433-443.
Yang, W.Z., Beauchemin, K.A. and Rode, L.M. 1999. Effects of an enzyme feed additive on extent of digestion and milk production of lactating dairy cows. J. Dairy Sci. 82: 391-403.
Zilio, E.M., Del Valle, T.A., Ghizzi, L.G., Takiya, C.S., Dias, M.S., Nunes, A.T., Silva, G.G. and Rennó, F.P. 2019.Effects of exogenous fibrolytic and amylolytic enzymes on ruminal fermentation and performance of mid-lactation dairy cows. J. Dairy Sci. 102: 4179-4189
© 2022 Michael et al. This is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Michael, N., George, S.K., Ally, K., Syam Mohan, K.M. and Gleeja, V.L.2022. Effect of supplementation of exogenous fibrolytic enzymes on milk yield and composition in early lactating crossbred dairy cows. J. Vet. Anim. Sci. 53 (3): 471-477
DOI: https://doi.org/10.51966/jvas.2022.53.3.471-477