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Journal of Veterinary and Animal Sciences

Volume: 53 Issue: 3

  • Open Access
  • Research Article

Effects of fermented soybean meal, probiotics and organic acids on intestinal morphology, pH and microbial count in broilers

Ratna Supriya1*, Binoj Chacko2, P. Anitha1, C. S. Suja1 and P. M. Priya3

1. Department of Poultry Science, College of Veterinary and Animal Sciences, Mannuthy, Thrissur-680651

2. University Poultry and Duck farm

3. Department of Veterinary Microbiology

Kerala Veterinary and Animal Sciences University, Kerala, India.

*Corresponding author: [email protected], Ph: 9182670331

Year: 2022, Page: 348-355, Doi: https://doi.org/10.51966/jvas.2022.53.3.348-355

Received: Dec. 15, 2021 Accepted: Jan. 5, 2022 Published: Sept. 30, 2022

Abstract

The study was conducted to compare the effects of fermented soybean meal (FSBM), probiotics and organic acids in the diet of broiler chicken on intestinal morphology, pH and microbial count. A total of 160, day-old Vencobb 430Y broiler chicks were randomly selected and divided into five treatments with four replicates of eight birds each.The dietary treatment groups were as follows: T1 - basal diet with corn-soybean meal (control); T2 - control diet with 100 per cent replacement of soybean meal by FSBM;T3 - control diet supplemented with 0.05 per cent probiotics;T4 - control diet supplemented with 0.1 per cent organic acids; T5 - control diet supplemented with both probiotics and organic acids. The results indicated significant (p<0.01) improvement in the duodenal and jejunal morphology of birds in terms of villi height, crypt depth and their ratio in all the experimental groups. There was significant (p<0.01) reduction in ileal E. coli and coliform count in broilers of all the experimental groups compared to the control group. All the experimental diets significantly increased (p<0.01) the lactic acid bacteria count in ileum of broilers. Ileal pH was significantly (p<0.01) lowered in all the experimental groups compared to control group. In this study, the dietary inclusion of FSBM showed better improvement in intestinal morphology and microbial balance compared to control, probiotic and organic acids supplemented groups and at par with that of combination of probiotic and organic acids group.

Keywords: Fermented soybean meal, intestinal morphology, probiotics, organic acids

References

AOAC. 2012. Official methods of analysis of AOAC International. (19th Ed.) Association of Official Analytical Chemists, Gaitherburg, Maryland, USA.

  Ashayerizadeh, A., Dastar, B., Shargh, M.S., Mahoonak, A.S. and Zerehdaran, S. 2018. Effects of feeding fermented rapeseed meal on growth performance, gastrointestinal microflora population, blood metabolites, meat quality, and lipid metabolism in broiler chickens. Livestock Sci. 216: 183-190.

 BIS, 2007. Poultry Feeds Specification. (5th Ed.). Bureau of Indian Standards, New Delhi, pp. 3-5.

 Dhama, K., Mahendran, M., Tomar, S. and Chauhan, R.S. 2008. Beneficial effects of probiotics and prebiotics in livestock and poultry: the current perspectives. Intas Polivet. 9: 1-12.

 Feng, J., Liu, X., Xu, Z.R., Wang, Y.Z. and Liu, J.X. 2007. Effects of fermented soybean meal on digestive enzyme activities and intestinal morphology in broilers. Poult. Sci. 86: 1149-1154.

 Fonseca, B.B., Beletti, M.E., Silva, M.S.D., Silva, P.L.D., Duarte, I.N. and Rossi, D.A. 2010. Microbiota of the cecum, ileum morphometry, pH of the crop and performance of broiler chickens supplemented with probiotics. Rev. Bras. de Zootec. 39: 1756-1760.

 Gridley, M.F. 1960. Manual of histologic and special staining techniques McGraw-Hill INC, New York.

 Hassanein, S.M. and Soliman, N.K. 2010. Effect of probiotic (Saccharomyces cerevisiae) adding to diets on intestinal microflora and performance of Hy-Line layers hens. J. Anim. Sci6: 159-169.

 Hung, A.T., Lin, S.Y., Yang, T.Y., Chou, C.K., Liu, H.C., Lu, J.J., Wang, B., Chen, S.Y. and Lien, T.F. 2012. Effects of Bacillus coagulans ATCC 7050 on growth performance, intestinal morphology, and microflora composition in broiler chickens. Anim. Prod. Sci. 52: 874-879.

Izat, A.L., Tidwell, N.M., Thomas, R.A., Reiber, M.A., Adams, M.H., Colberg, M. and Waldroup, P.W. 1990. Effects of a buffered propionic acid in diets on the performance of broiler chickens and on microflora of the intestine and carcass. Poult. Sci. 69: 818-826.

Jazi, V., Ashayerizadeh, A., Toghyani, M., Shabani, A. and Tellez, G. 2018. Fermented soybean meal exhibits probiotic properties when included in Japanese quail diet in replacement of soybean meal. Poult. Sci. 98: 2113-2122.

Jazi, V., Boldaji, F., Dastar, B., Hashemi, S.R. and Ashayerizadeh, A. 2017. Effects of fermented cottonseed meal on the growth performance, gastrointestinal microflora population and small intestinal morphology in broiler chickens. Br. Poult. Sci. 58: 402-408.

Jazi, V., Mohebodini, H., Ashayerizadeh, A., Shabani, A. and Barekatain, R. 2019. Fermented soybean meal ameliorates Salmonella typhimurium infection in young broiler chickens. Poult. sci. 98: 5648-5660.

Markovic, R., Sefer, D., Krstic, M. and Petrujkic, B. 2009. Effect of different growth promoters on broiler performance and gut morphology. Arch. Med. Vet. 41: 163-169.

Paul, S.K., Halder, G., Mondal, M.K. and Samanta, G. 2007. Effect of organic acid salt on the performance and gut health of broiler chicken. J. Poult. Sci. 44: 389-395.

Rodjan, P., Soisuwan, K., Thongprajukaew, K., Theapparat, Y., Khongthong, S., Jeenkeawpieam, J. and Salaeharae, T. 2018. Effect of organic acids or probiotics alone or in combination on growth performance, nutrient digestibility, enzyme activities, intestinal morphology and gut microflora in broiler chickens. J. Anim. Physiol. Anim. Nutr. 102: 931-940.

Salehimanesh, A., Mohammadi, M. and Roostaei‐Ali Mehr, M. 2016. Effect of dietary probiotic, prebiotic and synbiotic supplementation on performance, immune responses, intestinal morphology and bacterial populations in broilers.  J. Anim. Physiol. Anim. Nutr. 100: 694-700.

Shi, C., Zhang, Y., Lu, Z. and Wang, Y. 2017. Solid-state fermentation of corn-soybean meal mixed feed with Bacillus subtilis and Enterococcus faecium for degrading antinutritional factors and enhancing nutritional value. J. Anim. Sci. Biotechnol. 8: 1-9.

Shirani, V., Jazi, V., Toghyani, M., Ashayerizadeh, A., Sharifi, F. and Barekatain, R. 2019. Pulicaria     gnaphalodes powder in broiler diets: consequences for performance, gut health, antioxidant enzyme activity, and fatty acid profile. Poult. Sci. 98: 2577-2587.

Sen, S., Ingale, S.L., Kim, Y.W., Kim, J.S., Kim, K.H., Lohakare, J.D., Kim, E.K., Kim, H.S., Ryu, M.H., Kwon, I.K. and Chae, B.J. 2012. Effect of supplementation of Bacillus subtilis LS 1-2 to broiler diets on growth performance, nutrient retention, caecal microbiology and small intestinal morphology. Res. Vet. Sci. 93: 264-268.

Soumeh, E.A., Mohebodini, H., Toghyani, M., Shabani, A., Ashayerizadeh, A. and Jazi, V. 2019. Synergistic effects of fermented soybean meal and mannan-oligosaccharide on growth performance, digestive functions, and hepatic gene expression in broiler chickens. Poult. Sci. 98: 6797-6807.

Terlabie, N.N., Sakyi-Dawson, E. and Amoa-Awua, W.K. 2006. The comparative ability of four isolates of Bacillus subtilis to ferment soybeans into dawadawa. Int. J. Food Microbiol. 106: 145-152.

  Thirumeigmanam, D., Swain, R.K., Mohanty, S.P. and Pati, P.K. 2006. Effect of dietary supplementation of organic acids on performance of broiler chicken.  Indian J. Anim. Nutr. 23: 34-40.

 

   Zhang, Z.F. and Kim, I.H. 2014. Effects of multi strain probiotics on growth performance, apparent ileal nutrient digestibility, blood characteristics, caecal microbial shedding, and excreta odour contents in broilers. Poult. Sci. 93: 364-370.

Cite this article

Supriya, R., Chacko, B., Anitha, P., Suja, C. S. and Priya, P. M. 2022. Effects of fermented soybean meal, probiotics and organic acids on intestinal morphology, pH and microbial count in broilers. J. Vet. Anim. Sci. 53(3): 348-355

DOI: https://doi.org/10.51966/jvas.2022.53.3.348-355

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