Volume: 54 Issue: 1
Year: 2023, Page: 91-97, Doi: https://doi.org/10.51966/jvas.2023.54.1.91-97
Received: Sept. 14, 2022 Accepted: Nov. 1, 2022 Published: March 31, 2023
Platelet rich plasma (PRP) therapy is an integral part of regenerative medicine as the platelets possess a good healing capacity owing to the presence of a wide variety of growth factors in the platelet granules found in the cytoplasm of the platelet. Autologous PRP was prepared from the blood of the patient itself, without any preservatives. Storage of PRP was one of the main hurdles of the treatment modality. During storage, the platelet counts may get reduced, undergo activation or get contaminated with bacteria as no preservatives are used in the preparation of autologous PRP. Cytological changes and microbial quality of the PRP during storage at 4o C and -20o C for seven days were analysed in this study. Reduction in platelet count and the chance of microbial contamination were less when autologous PRP was stored at -20°C compared to 4°C.
Keywords: Canine, autologous platelet rich plasma, PRP, double centrifugation method, storage changes
Alio, J.L., Abad, M., Artola, A., Rodriguez-Prats, J.L., Pastor, S. and Ruiz-Colecha, J. 2007. Use of autologous platelet rich plasma in the treatment of dormant corneal ulcers. Ophthalmol. 114: 1286-1293.
Andia, I., Perez-Valle, A., Amo, C.D. and Maffulli, N. 2020. Freeze-Drying of Platelet-Rich Plasma: The Quest for Standardization. Int. J. Mol. Sci. 21: 1-18.
Araki, J., Jona, M., Eto, H., Aoi, N., Kato, H., Suga, H., Doi, K., Yatomi, Y. and Yoshimura, K. 2012. Optimized preparation method of platelet-concentrated plasma and noncoagulating platelet-derived factor concentrates: maximization of platelet concentration and removal of fibrinogen. Tissue Eng. Part C Methods. 18: 176-185.
Arnoczky, S.P. and Shebani-Rad, S. 2013. The basic science of platelet rich plasma (PRP): what clinicians need to know. Sports Med. Arthrosc. 21: 180-185.
Bausset, O., Giraudo, L., Veran, J., Magalon, J., Coudreuse, J., Magalon, G., Dubois, C., Serratrice, N., Dignat-George, F.O., and Sabatier, F. 2012. Formulation and Storage of Platelet-Rich Plasma Homemade Product. Biores. Open Access. 1: 115-123.
Bielecki, T.M., Gazdzik, T.S., Arendt, J., Szczepanski, T., Krol, W. and Wielkoszynski, T. 2007. Antibacterial effect of autologous platelet gel enriched with growth factors and other active substances: an in vitro study. J. Bone Joint Surg. Br. 89: 417-420.
Drago, L., Bortolin, M., Vassena, C., Taschieri, S. and DelFabbro, M. 2013. Antimicrobial activity of pure platelet rich plasma against microorganisms isolated from oral cavity. BMC Microbiol. 13: 1-5.
Franklin, S.P., Garner, B.C. and Cook, J.L. 2015. Characteristics of canine platelet-rich plasma prepared with five commercially available systems. Am. J. Vet. Res. 76: 822-827.
Hussain, K., Awakan, V. and Assad, A. 2017. Storage-induced changes in haematologic parameters of blood. Int. J. Med. Lab. Res. 2: 1-6.
Johnson, L., Tan, S., Wood, B., Davis, A. and Marks, D.C. 2016. Refrigeration and cryopreservation of platelets differentially affect platelet metabolism and function: a comparison with conventional platelet storage conditions. Transfusion. 56: 1807-1818.
Kattlove, H.E. and Alexander, B. 1971. The effect of cold on platelets: cold induced platelet aggregation. Blood. 38: 39-48.
Laiju, M.P. 2022. Autologous platelet rich plasma for treatment of sole lesions and expression profile of associated biomarkers in dairy cattle. PhD thesis, Kerala Veterinary and Animal Sciences University, Pookode, 248p.
Lee, J.H., Kim, M.J., Ha, S.W. and Kim, H.K. 2016. Autologous platelet-rich plasma eye drops in the treatment of recurrent corneal erosions. Korean J. Ophthalmol. 30:101-107.
Lee, J.M. and Kang, J.S. 2016. Changes of haematological references depends on storage period and temperature conditions in rats and dogs. Lab. Anim. Res. 32: 241-248.
Marx, R.E., Carlson, E.R., Eichstaedt, R.M., Schimmele, S.R., Strauss, J.E. and Georgeff, K.R. 1998. Platelet-rich plasma: growth factor enhancement for bone grafts. Oral Surg. Oral Med. Oral Pathol. Oral Radiol. Endod. 85: 638-646.
Mittal, K. and Kaur, R. 2015. Platelet storage lesion: an update. Asian J. Transfus. Sci. 9: 1-3.
Schnabel, L.V., Mohammed, H.O., Miller, B.J., McDermott, W.G., Jacobson, M.S., Santangelo, K.S. and Fortier, L.A. 2007. Platelet rich plasma (PRP) enhances anabolic gene expression patterns in flexor digitorum superficialis tendons. J. Orthop. Res. 25: 230-240.
Shin, H.S., Woo, H.M. and Kang, B. 2017. Optimisation of a double centrifugation method for preparation of canine platelet rich plasma. BMC Vet. Res. 13: 1-8.
Simona, D.P., Chiara, C., Francesca, A., Rosario, P., Giusi, V. and Elisabetta, G. 2017. Platelet rich plasma eye drops: preparation, storage and clinical use in dogs and cats. Preliminary results. Arch. Vet. Sci. Technol. 104: 1-4.
Wen, Y.H., Lin, W.Y., Lin, C.J., Sun, Y.C., Chang, P.Y., Wang, H.Y., Lu, J.J., Yeh, W.L., Chiueh, T.S. 2018. Sustained or higher levels of growth factors in platelet-rich plasma during 7- day storage. Clin. Chim. Acta. 483: 89–93.
Wu, T.E., Chen, C.J., Hu, C.C. and Cheng, C.K. 2014. Three case reports: Easy to prepare autologous platelet rich plasma in the treatment of refractory corneal ulcers. Taiwan J. Ophthalmol. 2014: 1-4.
© 2023 Jennes et al. This is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Jennes,D., Ramankutty,S., Anoop,S., Madhavan Unny,N., Sudheesh,S.N. and Martin, J.K.D. 2023. Evaluation of keeping quality of canine platelet rich plasma under different storage conditions. J. Vet. Anim. Sci. 54(1):91-97
DOI: https://doi.org/10.51966/jvas.2023.54.1.91-97