Volume: 50 Issue: 1
Year: 2019, Page: 74-76,
Received: May 24, 2018 Accepted: June 11, 2018 Published: Jan. 1, 2019
Study was undertaken to assess the effectiveness of lyophilisation in the preservation and recovery of Aspergillus flavus NRRL 6513 strain. The stock culture with known level of Aflatoxin B1 production ability was periodically subcultured in potato dextrose agar at 15 days interval to maintain its viability. Skim milk solution was used for the preservation of stock culture. The fungal culture was lyophilised and vacuum packed. The lyophilised fungus was recovered and aflatoxins B1 level was checked again in maize culture. The post lyophilisation level of Aflatoxin B1 was comparable to the amount produced by stock culture.
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