Volume: 50 Issue: 2
Year: 2019, Page: 154-158,
Received: May 12, 2019 Accepted: May 30, 2019 Published: July 1, 2019
Natural biomaterials are gaining momentum in tissue engineering because of many reported cases of recurrence in ventral hernia, repaired with synthetic mesh materials. Collagen is the main component of the extracellular matrix of the abdominal wall and their distribution and ratio alter during hernia formation. Therefore, a differential staining method for evaluating collagen distribution after biomaterial implantation known as Herovici’s polychrome staining was employed in this study. Here, abdominal wall defects were created in rat models (six animals/ group) and grafted with jejunum-derived scaffold (JDS) and a reference material, Surgisis (C-SIS) scaffold. The implanted scaffold samples were retrieved at 4 weeks post-implantation and subjected to Herovici’s staining. Data analysis revealed a significant (p<0.05) and favorable type I: III collagen ratio in C-SIS group compared to JDS group. This study shows Herovici’s staining to be a better differential stain that can be used for studying the efficacy of newly introduced biomaterial for ventral hernia repair in human and veterinary patients.
Keywords: Biomaterials, ventral hernia repair, collagen, Herovici’s staining
Anilkumar, T.V., Vineetha, V.P.V., Revi, D., Muhamed, J. and Rajan, A. 2014. Biomaterial properties of cholecyst-derived scaffold recovered by a nondetergent/enzymatic method. J. Biomed. Mater. Res. B. Appl. Biomater. 102:1506-16.
Ayubi, F.S., Armstrong, P.J., Mattia, M.S. and Parker, D.M. 2008. Abdominal wall hernia repair: a comparison of Permacol and Surgisis grafts in a rat hernia model. Hernia. 12: 373-378.
Badylak, S.F. and Gilbert, T.W. 2008. Immune response to biologic scaffold materials. Semin. Immunol. 20(2): 109-116.
Bilsel, Y. and Abci, I. 2012.The search for ideal hernia repair; mesh materials and types. Int. J. Surg. 10(6): 317-321.
Fitzgerald, A.M., Kirkpatrick, J.J., Foo, I.T., Naylor, I.L. 1996. Human skin histology as demonstrated by Herovici's stain: a guide for the improvement of dermal substitutes for use with cultured keratinocytes? Burns. 22(3):200-2.
Franz, M.G. 2006. The biology of hernias and the abdominal wall. Hernia. 10(6): 462-471.
Henriksen, N.A., Yadete, D.H., Sorensen, L.T., Agren, M.S. and Jorgensen, L.N. 2011. Connective tissue alteration in abdominal wall hernia. Br. J. Surg. 98(2): 210-9.
Herovici, C. 1963. A polychrome stain for differentiating precollagen from collagen. Stain Technol. 38: 204–206.
Petter-Puchner, A. 2007. Adverse effects of porcine small intestine submucosa implants in experimental ventral hernia repair. Surg. Endosc. 21: 830-1.
Reing J.E., Zhang L, Myers-Irvin J, Cordero K.E., Freytes D.O., Heber-Katz, E., Bedelbaeva, K., McIntosh, D., Dewilde, A., Braunhut, S.J., Badylak, S.F. 2009. Degradation products of extracellular matrix affect cell migration and proliferation. Tissue Eng. A. 15:605-14.
Valentin, J.E., Stewart-Akers, A.M., Gilbert, T.W. and Badylak, S.F. 2009. Macrophage participation in the degradation and remodeling of extracellular matrix scaffolds. Tissue Eng. A. 15(7): 1687-94.
© 2019 Dhanush Krishna et al. This is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.