Journal of Veterinary and Animal Sciences

Volume: 55 Issue: 2

  • Open Access
  • Research Article

Probiotic potential assessment and enzyme profiling of indigenous Lactic Acid bacteria

K. Sneha1, S. V. Aparna1* and A. K. Beena1

1. Department of Dairy Microbiology Verghese Kurien Institute of Dairy and Food Technology, Mannuthy, Thrissur-680651 Kerala Veterinary and Animal Sciences University Kerala, India

*Corresponding author: [email protected], Ph. 7558067959

Year: 2024, Page: 402-412, Doi: https://doi.org/10.51966/jvas.2024.55.2.402-412

Received: Dec. 21, 2023 Accepted: Feb. 7, 2024 Published: June 30, 2024

Abstract

The present study focuses on evaluating the probiotic potential and enzyme profiling of indigenous lactic acid bacteria. The cultures used were Lactiplantibacillus plantarum, two Lacticaseibacillus rhamnosus, Lactobacillus acidophilus, and Limosilactobacillus fermentum. In vitro analysis of acid and bile tolerance, cell surface hydrophobicity, aggregation potential, bile salt hydrolase activity, antimicrobial activities, and safety assessment were done for the isolates. All the cultures exhibited varying degrees of probiotic attributes confirming the strain specificity. The isolate Lactobacillus acidophilus, displayed the highest antimicrobial activity against both E. coli and S. aureus with zones of clearance measuring 16.50±0.50 mm and 19.00±1.00 mm, respectively. Enzyme profiling of the isolates was done for 19 enzymes and the results revealed the presence of four distinct enzymes in all cultures: leucine arylamidase, valine arylamidase, β-galactosidase, and β-glucosidase.

Keywords: Lactic acid bacteria, GRAS status, probiotics, API ZYM profile, enzymes

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Cite this article

Sneha, K., Aparna, S. V. and Beena, A. K. 2024. Probiotic potential assessment and enzyme profiling of indigenous Lactic Acid bacteria. J. Vet. Anim. Sci. 55(2):402-412

DOI: https://doi.org/10.51966/jvas.2024.55.2.402-412

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