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Journal of Veterinary and Animal Sciences

Volume: 54 Issue: 1

  • Open Access
  • Research Article

Qualitative and quantitative phytochemical analysis of methanolic extract of Magnolia champaca leaves

R. Shankar1, Preethy John2*, A. R. Nisha1, S. Sujith1, R. Uma3, V. Keerthika4 and Archana Raj1

1. Department of Veterinary Pharmacology and Toxicology, College of Veterinary and Animal Sciences, Mannuthy, Thrissur- 680 651

2. Department of Veterinary Pharmacology and Toxicology, College of Veterinary and Animal Sciences, pookode, Wayanad 673 576

3. Department of Veterinary Biochemistry, College of Veterinary and Animal Sciences, Mannuthy, Thrissur- 680 651

4. Veterinary Assistant Surgeon, Department of Animal Husbandry, Dairying and Fisheries, Tamil Nadu

Kerala Veterinary and Animal Sciences University, Kerala, India

*Corresponding author: [email protected], Ph: 9446031275

Year: 2023, Page: 204-213, Doi: https://doi.org/10.51966/jvas.2023.54.1.204-213

Received: Nov. 8, 2022 Accepted: Dec. 21, 2022 Published: March 31, 2023

Abstract

Plants remain a vibrant source of diverse bioactive phytochemicals that are secondary metabolites protecting them from infections and predations. Magnolia champaca is reported to possess a multitude of phytochemicals. In the present study, the phytochemical constituents of the methanolic extract of Magnolia champaca leaves were analysed qualitatively and with gas chromatography-tandem mass spectrometry (GC-MS/MS). Fourier transform infrared (FTIR) spectroscopic analysis was performed to identify the chemical nature of the extract and to find structurally similar compounds. Preliminary phytochemical screening revealed the presence of alkaloids, flavonoids, terpenes, glycosides, phenols, steroids, tannins and saponins. FTIR spectroscopic analysis revealed structurally related compounds. GC-MS/MS analysis revealed the presence of 99 diverse compounds with varied biological activities, among which 1,2,4-butanetriol, n-hexadecanoic acid, cis vaccenic acid, phytol, trans longipinocarveol and caryophyllene oxide were found predominantly. Thus, the identification and characterisation of the phytochemicals in the extract favour the development of novel therapeutic agents.

Keywords: Magnolia champaca, GC-MS/MS, FTIR, phytochemicals, phytol, caryophyllene oxide

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Cite this article

Shankar,R., Preethy,J., Nisha, A. R., Sujith,S., Uma,R., Keerthika,V. and Archana,R. 2023. Qualitative and quantitative phytochemical analysis of methanolic extract of Magnolia champaca leaves. J. Vet. Anim. Sci. 54(1):204-213
DOI: https://doi.org/10.51966/jvas.2023.54.1.204-213

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